Author | Ribeiro, Ricardo Souza | |
Author | Ladeira, Luisa | |
Author | Rezende, Antonio Mauro | |
Author | Fontes, Cor Jesus Fernandes | |
Author | Carvalho, Luzia Helena | |
Author | Brito, Cristiana Ferreira Alves de | |
Access date | 2014-11-28T17:06:50Z | |
Available date | 2014-11-28T17:06:50Z | |
Document date | 2011 | |
Citation | RIBEIRO, Ricardo Souza et al. Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates. Memórias do Instituto Oswaldo Cruz, v. 106, Supl.1, p. 27-33, 2011. | pt_BR |
ISSN | 0074-0276 | |
URI | https://www.arca.fiocruz.br/handle/icict/9006 | |
Sponsorship | FAPEMIG/PROBIC, Rede Malária/Pronex-CNPq, MS/DECIT, FAPEMIG, FAPEMAT, FAPERJ CFAB | pt_BR |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz | pt_BR |
Rights | open access | pt_BR |
Title | Analysis of the genetic variability of PvMSP-3α among Plasmodium vivax in Brazilian field isolates | pt_BR |
Type | Article | pt_BR |
Abstract | Reliable molecular markers are essential for a better understanding of the molecular epidemiology ofPlasmodium vivax, which is a neglected human malaria parasite. The aim of this study was to analyze the genetic diversity of P. vivax isolates from the Brazilian Amazon using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the highly polymorphic merozoite surface protein-3alpha(PvMSP-3α) gene. To accomplish this, 60 isolates of P. vivax from different endemic areas in the Brazilian Amazon were collected. The PvMSP-3α gene was amplified by nested-PCR. Three major types of the PvMSP-3αlocus were detected at different frequencies: type A (68%), B (15%) and C (17%). A single sample showed two PCR fragments, which corresponded to infection with types A and C. PCR-RFLP analysis using the HhaI restriction enzyme for 52 isolates clearly identified 11 haplotypes, eight of which were from type A, two from type B and only one from type C. Seven other isolates did not show a clear pattern using PCR-RFLP. This result might be due to multiple clone infections. This study showed a high diversity of the PvMSP-3α gene among P. vivaxisolates from the Brazilian Amazon, but also indicated that the detection performance of PCR-RFLP of the PvMSP-3α gene may not be sufficient to detect multiple clone infections. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular. Horizonte, MG, Brasil | pt_BR |
Affilliation | Universidade Federal do Mato Grosso. Departamento de Clínica Médica. Cuiabá, MT, Brasil | pt_BR |
Affilliation | Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil | pt_BR |
Affilliation | Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil | pt_BR |
Subject | malaria | pt_BR |
Subject | Plasmodium vivax | pt_BR |
Subject | genetic variation | pt_BR |
Subject | antigenic variability | pt_BR |
Subject | MSP3 protein | pt_BR |
Subject | restriction fragment length polymorphism | pt_BR |