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https://www.arca.fiocruz.br/handle/icict/9006
ANALYSIS OF THE GENETIC VARIABILITY OF PVMSP-3α AMONG PLASMODIUM VIVAX IN BRAZILIAN FIELD ISOLATES
Plasmodium vivax
genetic variation
antigenic variability
MSP3 protein
restriction fragment length polymorphism
Author
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil
Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil
Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular. Horizonte, MG, Brasil
Universidade Federal do Mato Grosso. Departamento de Clínica Médica. Cuiabá, MT, Brasil
Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil
Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil
Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil
Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Parasitologia Celular e Molecular. Horizonte, MG, Brasil
Universidade Federal do Mato Grosso. Departamento de Clínica Médica. Cuiabá, MT, Brasil
Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil
Fundação Oswaldo. Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Malária. Horizonte, MG, Brasil
Abstract
Reliable molecular markers are essential for a better understanding of the molecular epidemiology ofPlasmodium vivax, which is a neglected human malaria parasite. The aim of this study was to analyze the genetic diversity of P. vivax isolates from the Brazilian Amazon using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the highly polymorphic merozoite surface protein-3alpha(PvMSP-3α) gene. To accomplish this, 60 isolates of P. vivax from different endemic areas in the Brazilian Amazon were collected. The PvMSP-3α gene was amplified by nested-PCR. Three major types of the PvMSP-3αlocus were detected at different frequencies: type A (68%), B (15%) and C (17%). A single sample showed two PCR fragments, which corresponded to infection with types A and C. PCR-RFLP analysis using the HhaI restriction enzyme for 52 isolates clearly identified 11 haplotypes, eight of which were from type A, two from type B and only one from type C. Seven other isolates did not show a clear pattern using PCR-RFLP. This result might be due to multiple clone infections. This study showed a high diversity of the PvMSP-3α gene among P. vivaxisolates from the Brazilian Amazon, but also indicated that the detection performance of PCR-RFLP of the PvMSP-3α gene may not be sufficient to detect multiple clone infections.
Keywords
malariaPlasmodium vivax
genetic variation
antigenic variability
MSP3 protein
restriction fragment length polymorphism
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