Author | Reis, Eliana Almeida Gomes | |
Author | Carmo, Theomira Mauadie de Azevedo | |
Author | McBride, Alan John Alexander | |
Author | Harn Junior, Donald A | |
Author | Reis, Mitermayer Galvão dos | |
Access date | 2014-04-25T18:33:00Z | |
Available date | 2014-04-25T18:33:00Z | |
Document date | 2007 | |
Citation | REIS, E. A. G. et al. Naïve donor responses to Schistosoma mansoni soluble egg antigens. Scandinavian Journal of Immunology , v.66, n. 6, p. 662–670, 2007. | pt_BR |
ISSN | 1365-3083 | |
URI | https://www.arca.fiocruz.br/handle/icict/7545 | |
Language | eng | pt_BR |
Publisher | Blackwell Publishing Ltd. | pt_BR |
Rights | open access | pt_BR |
Title | Naïve donor responses to Schistosoma mansoni soluble egg antigens. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1111/j.1365-3083.2007.02024.x | |
Abstract | Schistosome infection induces profound Th-biasing and immune suppression.
Although much has been examined in mice, few studies have examined
responses of naı¨ve humans to schistosome antigens. In this study, we examined
the response of naı¨ve human peripheral blood mononuclear cells (nPBMC) to
stimulation with Schistosoma mansoni soluble egg antigen (SEA) using a priming
in vitro (PIV) assay. We found that SEA induced a pronounced CD4+
T-helper cell response based on cytokine secretion and phenotyping markers.
SEA-stimulated nPBMC (SEA cells) at day 7 post-priming and after the first
recall consisted predominantly of Th0-like CD4+ T cells. Following the second
recall, the majority of donor (10 ⁄ 12) responses were Th2-like. The cell population
consisted of approximately 64% CD4+, 17% CD8+high, 12% CD19+, and
7% CD23+ cells. The CD4+ population also expressed HLA-DR+, CD54+,
CD45RO+ and CD25+ whereas the CD19+ cells expressed CD80 and CD86.
Following priming, we detected high levels of IL-6, IFN-c, IL-12p40, IL-10
and IL-5. Upon restimulation, SEA cells secreted IL-5 and high levels of
IL-10, typical of a Th2-like response. The data presented herein shows that the
majority of naı¨ve donor dendritic cells, following stimulation with SEA, prime
and clonally expand SEA-specific T cells towards a Th2-type response. However,
two donors responded with an atypical response, producing IFN-c coincident
with low levels of IL-10. Whether this differential response was due to HLA or
other genes was not determined but is currently under investigation. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Department of Immunology and Infectious Diseases. Harvard School of Public Health. Boston, MA, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Faculty of Medicine of Federal University of Bahia. Department of Anatomical Pathology and Legal Medicine. Salvador, BA, Brasil | pt_BR |
DeCS | Antígenos de Helmintos/administração & dosagem | pt_BR |
DeCS | Óvulo/imunologia | pt_BR |
DeCS | Schistosoma mansoni/imunologia | pt_BR |
DeCS | Esquistossomose mansoni/imunologia | pt_BR |
DeCS | Adulto | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Antígenos de Helmintos/imunologia | pt_BR |
DeCS | Linfócitos T CD4-Positivos/imunologia | pt_BR |
DeCS | Citocinas/imunologia | pt_BR |
DeCS | Células Dendríticas/parasitologia | pt_BR |
DeCS | Citometria de Fluxo | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Solubilidade | pt_BR |
DeCS | Células Th2/imunologia | pt_BR |
DeCS | Células Th2/parasitologia | pt_BR |