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https://www.arca.fiocruz.br/handle/icict/7545
NAÏVE DONOR RESPONSES TO SCHISTOSOMA MANSONI SOLUBLE EGG ANTIGENS.
Óvulo/imunologia
Schistosoma mansoni/imunologia
Esquistossomose mansoni/imunologia
Adulto
Animais
Antígenos de Helmintos/imunologia
Linfócitos T CD4-Positivos/imunologia
Citocinas/imunologia
Células Dendríticas/parasitologia
Citometria de Fluxo
Humanos
Solubilidade
Células Th2/imunologia
Células Th2/parasitologia
Author
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Department of Immunology and Infectious Diseases. Harvard School of Public Health. Boston, MA, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Faculty of Medicine of Federal University of Bahia. Department of Anatomical Pathology and Legal Medicine. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Department of Immunology and Infectious Diseases. Harvard School of Public Health. Boston, MA, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Faculty of Medicine of Federal University of Bahia. Department of Anatomical Pathology and Legal Medicine. Salvador, BA, Brasil
Abstract
Schistosome infection induces profound Th-biasing and immune suppression.
Although much has been examined in mice, few studies have examined
responses of naı¨ve humans to schistosome antigens. In this study, we examined
the response of naı¨ve human peripheral blood mononuclear cells (nPBMC) to
stimulation with Schistosoma mansoni soluble egg antigen (SEA) using a priming
in vitro (PIV) assay. We found that SEA induced a pronounced CD4+
T-helper cell response based on cytokine secretion and phenotyping markers.
SEA-stimulated nPBMC (SEA cells) at day 7 post-priming and after the first
recall consisted predominantly of Th0-like CD4+ T cells. Following the second
recall, the majority of donor (10 ⁄ 12) responses were Th2-like. The cell population
consisted of approximately 64% CD4+, 17% CD8+high, 12% CD19+, and
7% CD23+ cells. The CD4+ population also expressed HLA-DR+, CD54+,
CD45RO+ and CD25+ whereas the CD19+ cells expressed CD80 and CD86.
Following priming, we detected high levels of IL-6, IFN-c, IL-12p40, IL-10
and IL-5. Upon restimulation, SEA cells secreted IL-5 and high levels of
IL-10, typical of a Th2-like response. The data presented herein shows that the
majority of naı¨ve donor dendritic cells, following stimulation with SEA, prime
and clonally expand SEA-specific T cells towards a Th2-type response. However,
two donors responded with an atypical response, producing IFN-c coincident
with low levels of IL-10. Whether this differential response was due to HLA or
other genes was not determined but is currently under investigation.
DeCS
Antígenos de Helmintos/administração & dosagemÓvulo/imunologia
Schistosoma mansoni/imunologia
Esquistossomose mansoni/imunologia
Adulto
Animais
Antígenos de Helmintos/imunologia
Linfócitos T CD4-Positivos/imunologia
Citocinas/imunologia
Células Dendríticas/parasitologia
Citometria de Fluxo
Humanos
Solubilidade
Células Th2/imunologia
Células Th2/parasitologia
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