Author | Campos, Letícia Bomfim | |
Author | Almeida, Nathália Alves Araújo de | |
Author | Santana, Catarina Góis de | |
Author | Barbosa, Evorah Nascimento Pereira | |
Author | Horta, Marco Aurelio Pereira | |
Author | Pires, Márcia Amendola | |
Author | Mello, Carlos Eduardo Brandão | |
Author | Paula, Vanessa Salete de | |
Author | Barros, José Júnior França de | |
Access date | 2023-04-12T11:28:26Z | |
Available date | 2023-04-12T11:28:26Z | |
Document date | 2023 | |
Citation | CAMPOS, Letícia Bomfim et al. Before Direct-Acting Antivirals for Hepatitis C Virus: Evaluation of Core Protein R70Q and L/C91M Substitutions in Chronically Infected Brazilian Patients Unresponsive to IFN and/or RBV. Viruses, v. 15, 187, p. 1 - 12, Jan. 2023. | en_US |
ISSN | 1999-4915 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/57743 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | |
Subject in Portuguese | Hepatite C crônica | en_US |
Subject in Portuguese | Resposta viral não sustentada | en_US |
Subject in Portuguese | Substituições de aminoácidos R70Q/H e L/C91M | en_US |
Subject in Portuguese | Perfil clínico e epidemiológico | en_US |
Subject in Portuguese | Fatores de risco do hospedeiro | en_US |
Subject in Portuguese | Subpopulações | en_US |
Subject in Portuguese | Pirosequenciamento | en_US |
Title | Before Direct-Acting Antivirals for Hepatitis C Virus: Evaluation of Core Protein R70Q and L/C91M Substitutions in Chronically Infected Brazilian Patients Unresponsive to IFN and/or RBV | en_US |
Type | Article | |
DOI | 10.3390/v15010187 | |
Abstract | Although chronic hepatitis C has been effectively treated with direct-acting antivirals
(DAAs), the use of conventional therapy with peg-interferon (Peg-IFN) or (predominantly) ribavirin
(RBV), remains widespread. R70Q/H and L/C91M amino acid substitutions in the hepatitis C virus
(HCV) core protein may modulate responses to IFN and/or RBV, and are associated with cirrhosis,
hepatocellular carcinoma (HCC), insulin resistance, and liver steatosis. We evaluated the R70Q/H
and L/C91M substitutions, clinical and epidemiological profiles, and risk factors of Brazilian patients
chronically infected with HCV subgenotypes 1a and 1b (HCV-GT1a and HCV-GT1b) unresponsive
to IFN and/or RBV therapy. Sequencing and pyrosequencing analyses and sociodemographic and
clinical predictive variables were used to assess the relationship between R70Q/H and L/C91M
substitutions. Leukocyte counts, ALT levels, and ALT/AST ratios were significantly reduced in
treated individuals, but more of these patients had advanced fibrosis and cirrhosis. L91M was more
prevalent (19.7%), occurring only in HCV-GT1b, followed by R70Q/P (11.5%) and R70P (1.4%).
R70Q/P exhibited higher mean AST, ALT, and GGT values, whereas L91M showed higher mean GGT
values. Pyrosequencing of the L91M position revealed mutant subpopulations in 43.75% of samples. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Feferal do Estado do Rio de Janeiro. Hospital Universitário Gaffre e Guinle. Ambulatório de Doenças de Fígado. Rio de janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. BSL-3 Facility. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Feferal do Estado do Rio de Janeiro. Hospital Universitário Gaffre e Guinle. Ambulatório de Doenças de Fígado. Rio de janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Feferal do Estado do Rio de Janeiro. Hospital Universitário Gaffre e Guinle. Ambulatório de Doenças de Fígado. Rio de janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Chronic hepatitis C | en_US |
Subject | Non-sustained viral response | en_US |
Subject | Amino acid substitutions R70Q/H and L/C91M | en_US |
Subject | Clinical and epidemiological profiles | en_US |
Subject | Host risk factors | en_US |
Subject | Subpopulations | en_US |
Subject | Pyrosequencing | en_US |