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MOLECULAR CHARACTERIZATION OF PAENIBACILLUS ANTARCTICUS IPAC21, A BIOEMULSIFIER PRODUCER ISOLATED FROM ANTARCTIC SOIL
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Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology. Thuwal, Saudi Arabia.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology. Thuwal, Saudi Arabia.
Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
Abstract
Paenibacillus antarcticus IPAC21, an endospore-forming and bioemulsifierproducing
strain, was isolated from King George Island, Antarctica. As
psychrotolerant/psychrophilic bacteria can be considered promising sources
for novel products such as bioactive compounds and other industrially relevant
substances/compounds, the IPAC21 genome was sequenced using Illumina
Hi-seq, and a search for genes related to the production of bioemulsifiers and
other metabolic pathways was performed. The IPAC21 strain has a genome of
5,505,124 bp and a G + C content of 40.5%. Genes related to the biosynthesis of
exopolysaccharides, such as the gene that encodes the extracellular enzyme
levansucrase responsible for the synthesis of levan, the 2,3-butanediol pathway,
PTS sugar transporters, cold-shock proteins, and chaperones were found in its
genome. IPAC21 cell-free supernatants obtained after cell growth in trypticase
soy broth at different temperatures were evaluated for bioemulsifier production
by the emulsification index (EI) using hexadecane, kerosene and diesel. EI values
higher than 50% were obtained using the three oil derivatives when IPAC21 was
grown at 28°C. The bioemulsifier produced by P. antarcticus IPAC21 was stable
at different NaCl concentrations, low temperatures and pH values, suggesting
its potential use in lower and moderate temperature processes in the petroleum
industry.
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