Author | Oliveira, Carlos Rocha | |
Author | Bella, Leonardo Mendes | |
Author | Duarte, Marília Cristina | |
Author | Vieira, Rodolfo de Paula | |
Access date | 2022-01-16T20:44:35Z | |
Available date | 2022-01-16T20:44:35Z | |
Document date | 2021 | |
Citation | OLIVEIRA, Carlos Rocha et al. Brazilian essential oil of Cymbopogon martini (Roxb.) Will. Watson (Poaceae): positive effects on inflammation-induced human fibroblasts and skin aging. Revista Fitos, v. 15, n. 4, p. 444-455, Dec. 2021. | en_US |
ISSN | 1808-9569 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/50772 | |
Language | eng | en_US |
Publisher | Fundação Oswaldo Cruz. Farmanguinhos. Núcleo de Gestão em Biodiversidade e Saúde. | en_US |
Rights | open access | |
Title | Brazilian essential oil of Cymbopogon martini (Roxb.) Will. Watson (Poaceae): positive effects on inflammation-induced human fibroblasts and skin aging | en_US |
Type | Article | en_US |
DOI | 10.32712/2446-4775.2021.1127 | |
Abstract | The study evaluated the effects of essential oil from Cymbopogon martini (Roxb.) Will. Watson (CMEO) on lipopolysaccharide (LPS)-stimulated human fibroblasts. Samples were collected in Monte Verde, Minas Gerais, Brazil and analyzed by gas chromatography with mass spectrometry. The fibroblasts were cultured in a monolayer using Iscove's medium and stimulated by LPS (1 μg/mL) and incubated for 24 h at 37°C. The cytotoxicity was evaluated by MTT assay and collagen concentration by Sirius red. Collagenase activity, hyaluronic acid, and the concentrations of IL-1β; IL-6; MCP-1 (CCL2), and MIP-1-α (CCL3) were evaluated. The effect of CMEO on the expression of mRNA and the secretion of enzymes in fibroblasts were evaluated by RT-qPCR and ELISA, respectively. CMEO was cytotoxic against fibroblasts, in which 10 μg/mL inhibited 50% of cell viability. When treated with CMEO, the fibroblasts produced more collagen and hyaluronic acid than control cells. When stimulated by LPS, fibroblasts exhibited higher production of IL-6, IL-1β, MCP-1, and MIP-1α than control cells. The study demonstrated the effects of CMEO on the modulation of mediators related to inflammation and decreasing the mRNA and secretion levels of metalloproteinases, revealing to be a promising candidate for anti-aging effects and wound healing treatments. | en_US |
Affilliation | Universidade Anhembi Morumbi. Ciências Biológicas e da Saúde. São Paulo, SP, Brasil. | |
Affilliation | Universidade Anhembi Morumbi. Ciências Biológicas e da Saúde. São Paulo, SP, Brasil. | |
Affilliation | Universidade de Mogi das Cruzes. Centro de Ciências Biomédicas. Laboratório de Sistemática Vegetal. Mogi das Cruzes, SP, Brasil. | |
Affilliation | Universidade Brasil. São Paulo, SP, Brasil. | |
Subject | Cymbopogon martini | en_US |
Subject | Anti-inflammatory | en_US |
Subject | Anti-aging | en_US |
Subject | Hyaluronic acid | en_US |
Subject | Collagen | en_US |
e-ISSN | 2446-4775 | |