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https://www.arca.fiocruz.br/handle/icict/50772
BRAZILIAN ESSENTIAL OIL OF CYMBOPOGON MARTINI (ROXB.) WILL. WATSON (POACEAE): POSITIVE EFFECTS ON INFLAMMATION-INDUCED HUMAN FIBROBLASTS AND SKIN AGING
Author
Affilliation
Universidade Anhembi Morumbi. Ciências Biológicas e da Saúde. São Paulo, SP, Brasil.
Universidade Anhembi Morumbi. Ciências Biológicas e da Saúde. São Paulo, SP, Brasil.
Universidade de Mogi das Cruzes. Centro de Ciências Biomédicas. Laboratório de Sistemática Vegetal. Mogi das Cruzes, SP, Brasil.
Universidade Brasil. São Paulo, SP, Brasil.
Universidade Anhembi Morumbi. Ciências Biológicas e da Saúde. São Paulo, SP, Brasil.
Universidade de Mogi das Cruzes. Centro de Ciências Biomédicas. Laboratório de Sistemática Vegetal. Mogi das Cruzes, SP, Brasil.
Universidade Brasil. São Paulo, SP, Brasil.
Abstract
The study evaluated the effects of essential oil from Cymbopogon martini (Roxb.) Will. Watson (CMEO) on lipopolysaccharide (LPS)-stimulated human fibroblasts. Samples were collected in Monte Verde, Minas Gerais, Brazil and analyzed by gas chromatography with mass spectrometry. The fibroblasts were cultured in a monolayer using Iscove's medium and stimulated by LPS (1 μg/mL) and incubated for 24 h at 37°C. The cytotoxicity was evaluated by MTT assay and collagen concentration by Sirius red. Collagenase activity, hyaluronic acid, and the concentrations of IL-1β; IL-6; MCP-1 (CCL2), and MIP-1-α (CCL3) were evaluated. The effect of CMEO on the expression of mRNA and the secretion of enzymes in fibroblasts were evaluated by RT-qPCR and ELISA, respectively. CMEO was cytotoxic against fibroblasts, in which 10 μg/mL inhibited 50% of cell viability. When treated with CMEO, the fibroblasts produced more collagen and hyaluronic acid than control cells. When stimulated by LPS, fibroblasts exhibited higher production of IL-6, IL-1β, MCP-1, and MIP-1α than control cells. The study demonstrated the effects of CMEO on the modulation of mediators related to inflammation and decreasing the mRNA and secretion levels of metalloproteinases, revealing to be a promising candidate for anti-aging effects and wound healing treatments.
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