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https://www.arca.fiocruz.br/handle/icict/49847
BONE MARROW MONONUCLEAR CELL TRANSPLANT PREVENTS RAT DEPRESSION AND MODULATES INFLAMMATORY AND NEUROGENIC MOLECULES
Medula óssea
Tansplantação
Inflamação
Depressão
Tratamento
Author
Costa-Ferro, Zaquer Suzana Munhoz
Lima, Pedro Antônio Schmidt do Prado
Onsten, Guilherme Ary
Oliveira, Gutierre Neves
Brito, Guilherme Camargo
Ghilardi, Isadora Machado
Santos, Paula Gabrielli dos
Bertinatto, Ricardo Jean
Silva, Daniele Vieira da
Salamoni, Simone Denise
Machado, Denise Cantarelli
Cruz, Ivana Beatrice Mânica da
Souza, Bruno Solano de Freitas
Costa, Jaderson Costa da
Lima, Pedro Antônio Schmidt do Prado
Onsten, Guilherme Ary
Oliveira, Gutierre Neves
Brito, Guilherme Camargo
Ghilardi, Isadora Machado
Santos, Paula Gabrielli dos
Bertinatto, Ricardo Jean
Silva, Daniele Vieira da
Salamoni, Simone Denise
Machado, Denise Cantarelli
Cruz, Ivana Beatrice Mânica da
Souza, Bruno Solano de Freitas
Costa, Jaderson Costa da
Affilliation
"Múltipla ver em Notas"
Abstract
Introduction: Major depressive disorder is associated with chronic inflammation and deficient production of
brain-derived neurotrophic factor (BDNF). Bone marrow mononuclear cell (BMMC) transplantation has an antiinflammatory
effect and has been proven effective in restoring non-depressive behavior. This study investigated
whether BMMC transplantation can prevent the development of depression or anxiety in chronic mild stress
(CMS), as well as its effect on inflammatory and neurogenic molecules.
Method: Three groups of animals were compared: BMMC-transplanted animals subjected to CMS for 45 days,
CMS non-transplanted rats, and control animals. After the CMS period, the three groups underwent the following
behavioral tests: sucrose preference test (SPT), eating-related depression test (ERDT), social avoidance test (SAT),
social interaction test (SIT), and elevated plus maze test (EPMT). Transplanted cell tracking and measurement of
the expression of high-mobility group box 1 (HMGB1), interleukin-1β (IL-1β), tumor necrosis factor (TNFα), and
BDNF were performed on brain and spleen tissues.
Results: BMMC transplantation prevented the effects of CMS in the SPT, ERDT, SAT, and SIT, while prevention
was less pronounced in the EPMT. It was found to prevent increased HMGB-1 expression induced by CMS in the
hippocampus and spleen, increase BDNF expression in both tissues, and prevent increased IL-1β expression in the
hippocampus alone, while no effect of the transplant was observed in the TNFα expression. In addition, no
transplanted cells were found in either the brain or spleen.
Conclusions: BMMC transplantation prevents the development of depression and anxiety-like behavior triggered
by CMS. It could prevent increased HMGB-1 and IL-1β expression in the hippocampus and increased BDNF
expression in the same tissue. Cell treatment represents a further perspective in the research and treatment of
depression and possible mood disorders.
Keywords in Portuguese
Células mononuclearesMedula óssea
Tansplantação
Inflamação
Depressão
Tratamento
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