Author | Cordeiro, Taps Aparecida Reis | |
Author | Martins, Helen Rodrigues | |
Author | Franco, Diego Leoni | |
Author | Santos, Fred Luciano Neves | |
Author | Celedon, Paola Alejandra Fiorani | |
Author | Cantuaria, Vinicius Lopes | |
Author | Lana, Marta de | |
Author | Reis, Alexandre Barbosa | |
Author | Ferreira, Lucas Franco | |
Access date | 2020-10-28T12:53:03Z | |
Available date | 2020-10-28T12:53:03Z | |
Document date | 2020 | |
Citation | CORDEIRO, Taps Aparecida Reis et al. Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis. Biosensors and Bioelectronics, 2020. | pt_BR |
ISSN | 0956-5663 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44242 | |
Sponsorship | Fundação de Amparo a Pesquisa do
Estado de Minas Gerais (FAPEMIG), Brazil, (Project: APQ 03097 15). | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Chagas | pt_BR |
Subject in Portuguese | Leishmaniose visceral | pt_BR |
Subject in Portuguese | Detecção dupla | pt_BR |
Subject in Portuguese | Imunossensor impedimétrico | pt_BR |
Subject in Portuguese | Eletropolimerização | pt_BR |
Title | Impedimetric immunosensor for rapid and simultaneous detection of chagas and visceral leishmaniasis for point of care diagnosis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.bios.2020.112573 | |
Abstract | In this work, a dual detection system based on an impedimetric immunosensor was developed for the lust time
for the simultaneous detection of anti-Tgpanosoma cruzi and anti-Leishmania infanturn antibodies in human and
dog serum samples. The IBMP 8.1 and rLcilA/rLci2B recombinant antigens were inunobilized over the surface of
dual screen-printed carbon electrodes (W1 and W2) modified with poly (4-hydroxyphenylacetic acid). Under
optimized conditions, the immunosensor recognized specific interactions for anti-T. cruzi antibodies up to a
dilution of 1:10,240 and for anti-L. infant-tun up to 1:5120 in canine seams samples. Relative standard deviation
(RSD) values of 2.8% for W1 and 3.6% for W2 were obtained for T. cruzi (W1) and L infannun antigen (W2)
samples in three different electrodes for 3 days (n = 9). The immunosensor was stored at 4 °C for 8 weeks, with
activity retention of 70.2% in W1 and 78.2% in W2. The results using the recombinant proteins revealed that all
antigens discriminated between negative and positive samples (p < 0.0001) in both dog and human groups, as
well as no cross-reactivity could be detected among sera with other infections. With this approach,
immunosensor-based diagnostic tests achieved 100% accuracy, suggesting that the antigens are eligible to enter
Phase-II studies. | pt_BR |
Subject | Chagas | pt_BR |
Subject | Visceral leishmaniasis | pt_BR |
Subject | Dual detection | pt_BR |
Subject | Impedimetric immunosensor | pt_BR |
Subject | Electropolymerization | pt_BR |