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https://www.arca.fiocruz.br/handle/icict/44242
IMPEDIMETRIC IMMUNOSENSOR FOR RAPID AND SIMULTANEOUS DETECTION OF CHAGAS AND VISCERAL LEISHMANIASIS FOR POINT OF CARE DIAGNOSIS
Leishmaniose visceral
Detecção dupla
Imunossensor impedimétrico
Eletropolimerização
Visceral leishmaniasis
Dual detection
Impedimetric immunosensor
Electropolymerization
Author
Abstract
In this work, a dual detection system based on an impedimetric immunosensor was developed for the lust time
for the simultaneous detection of anti-Tgpanosoma cruzi and anti-Leishmania infanturn antibodies in human and
dog serum samples. The IBMP 8.1 and rLcilA/rLci2B recombinant antigens were inunobilized over the surface of
dual screen-printed carbon electrodes (W1 and W2) modified with poly (4-hydroxyphenylacetic acid). Under
optimized conditions, the immunosensor recognized specific interactions for anti-T. cruzi antibodies up to a
dilution of 1:10,240 and for anti-L. infant-tun up to 1:5120 in canine seams samples. Relative standard deviation
(RSD) values of 2.8% for W1 and 3.6% for W2 were obtained for T. cruzi (W1) and L infannun antigen (W2)
samples in three different electrodes for 3 days (n = 9). The immunosensor was stored at 4 °C for 8 weeks, with
activity retention of 70.2% in W1 and 78.2% in W2. The results using the recombinant proteins revealed that all
antigens discriminated between negative and positive samples (p < 0.0001) in both dog and human groups, as
well as no cross-reactivity could be detected among sera with other infections. With this approach,
immunosensor-based diagnostic tests achieved 100% accuracy, suggesting that the antigens are eligible to enter
Phase-II studies.
Keywords in Portuguese
ChagasLeishmaniose visceral
Detecção dupla
Imunossensor impedimétrico
Eletropolimerização
Keywords
ChagasVisceral leishmaniasis
Dual detection
Impedimetric immunosensor
Electropolymerization
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