Author | Britto, Constança | |
Author | Silveira, Celeste | |
Author | Cardoso, Maria Angelica | |
Author | Marques, Patricia | |
Author | Luquetti, Alejandro | |
Author | Macêdo, Vanize | |
Author | Fernandes, Octávio | |
Access date | 2020-10-23T19:43:20Z | |
Available date | 2020-10-23T19:43:20Z | |
Document date | 2001 | |
Citation | BRITTO, Constança et al. Parasite Persistence in Treated Chagasic Patients Revealed by Xenodiagnosis and Polymerase Chain Reaction. Memórias do instituto Oswaldo Cruz, Rio de Janeiro, v. 96, 4p, 2001. | pt_BR |
ISSN | 0074-0276 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/44152 | |
Language | eng | pt_BR |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Subject in Portuguese | Doença de Chagas | pt_BR |
Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
Subject in Portuguese | Tratamento | pt_BR |
Subject in Portuguese | DNA de cinetoplasto | pt_BR |
Title | Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction | pt_BR |
Type | Article | pt_BR |
Abstract | Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas
disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and
48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the
acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%.
In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative
results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which
36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals
had their antibodies titers decreased to such a level that the final results were considered as doubtful
and 16 of them presented negative PCR. The molecular method represents a clear advantage over
conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent
chemotherapy. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade de Brasília. Departamento de Medicina Tropical. Brasília, DF, Brasil. | pt_BR |
Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil. | pt_BR |
Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil. | pt_BR |
Affilliation | Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil. | pt_BR |
Affilliation | Universidade de Brasília. Departamento de Medicina Tropical. Brasília, DF, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Trypanosoma cruzi | pt_BR |
Subject | kinetoplast DNA | pt_BR |
Subject | Chagas disease | pt_BR |
Subject | PCR | pt_BR |
Subject | Treatment | pt_BR |
e-ISSN | 1678-8060 | |