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PARASITE PERSISTENCE IN TREATED CHAGASIC PATIENTS REVEALED BY XENODIAGNOSIS AND POLYMERASE CHAIN REACTION
Doença de Chagas
Reação em Cadeia da Polimerase
Tratamento
DNA de cinetoplasto
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Universidade de Brasília. Departamento de Medicina Tropical. Brasília, DF, Brasil.
Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.
Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.
Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.
Universidade de Brasília. Departamento de Medicina Tropical. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil.
Universidade de Brasília. Departamento de Medicina Tropical. Brasília, DF, Brasil.
Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.
Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.
Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia, Go, Brasil.
Universidade de Brasília. Departamento de Medicina Tropical. Brasília, DF, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Rio de Janeiro, RJ, Brasil.
Abstract
Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas
disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and
48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the
acute phase led to PCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%.
In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative
results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which
36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals
had their antibodies titers decreased to such a level that the final results were considered as doubtful
and 16 of them presented negative PCR. The molecular method represents a clear advantage over
conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent
chemotherapy.
Keywords in Portuguese
Trypanosoma cruziDoença de Chagas
Reação em Cadeia da Polimerase
Tratamento
DNA de cinetoplasto
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