Author | Kikuti, Mariana | |
Author | Tauro, Laura Beatriz | |
Author | Moreira, Patrícia Sousa dos Santos | |
Author | Nascimento, Leile Camila Jacob | |
Author | Portilho, Moyra Machado | |
Author | Soares, Gúbio C. | |
Author | Weaver, Scott C. | |
Author | Reis, Mitermayer Galvão dos | |
Author | Kitron, Uriel | |
Author | Ribeiro, Guilherme de Sousa | |
Access date | 2019-12-10T17:51:29Z | |
Available date | 2019-12-10T17:51:29Z | |
Document date | 2020 | |
Citation | KIKUTI, Mariana et al. Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. International Journal of Infectious Diseases, v. 91, p. 38-43, 2020. | pt_BR |
ISSN | 1201-9712 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/37810 | |
Sponsorship | Brazilian National Council for
Scientific and Technological Development (grants 400830/2013-2
and 440891/2016-7 to GSR; and scholarships to UK, MGR, and
GSR); the Bahia Foundation for Research Support (grants PET0026/
2013, APP0044/2016, and PET0022/2016 to GSR); the Coordination
for the Improvement of Higher Education Personnel, Brazilian
Ministry of Education (grant 88887.130746/2016-00 to GSR and
scholarship to MK); the Oswaldo Cruz Foundation; the Federal
University of Bahia; and the Department of Science and
Technology, Secretariat of Science, Technology and Strategic
Inputs, Brazilian Ministry of Health. | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Precisão | pt_BR |
Subject in Portuguese | Sensibilidade e especificidade | pt_BR |
Subject in Portuguese | Desempenho de diagnóstico | pt_BR |
Subject in Portuguese | Vírus Chikungunya | pt_BR |
Subject in Portuguese | Ensaio de imunoabsorção enzimática | pt_BR |
Subject in Portuguese | ELISA | pt_BR |
Title | Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.ijid.2019.11.001 | |
Abstract | To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness
surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous
CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and
paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days postonset
of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases,
and on negative patients.
Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for
convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for
acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were
88.5% and 83.9%, respectively.
Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun
test returned more equivocal results and presented a slightly lower specificity, which might result in a
higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of
CHIKV infection is lower. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Biología Subtropical. Nodo Iguazú. Puerto Iguazu, Misiones, Argentina. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Universidade Federal da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | University of Texas Medical Branch. Galveston, TX, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Emory University. Atlanta, GA, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil. | pt_BR |
Subject | Accuracy | pt_BR |
Subject | Sensitivity and specificity | pt_BR |
Subject | Diagnostic performance | pt_BR |
Subject | Chikungunya virus | pt_BR |
Subject | Enzyme-linked immunosorbent assay | pt_BR |
Subject | ELISA | pt_BR |