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https://www.arca.fiocruz.br/handle/icict/37810
EVALUATION OF TWO COMMERCIALLY AVAILABLE CHIKUNGUNYA VIRUS IGM ENZYME-LINKED IMMUNOASSAYS (ELISA) IN A SETTING OF CONCOMITANT TRANSMISSION OF CHIKUNGUNYA, DENGUE AND ZIKA VIRUSES
Sensibilidade e especificidade
Desempenho de diagnóstico
Vírus Chikungunya
Ensaio de imunoabsorção enzimática
ELISA
Sensitivity and specificity
Diagnostic performance
Chikungunya virus
Enzyme-linked immunosorbent assay
ELISA
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Biología Subtropical. Nodo Iguazú. Puerto Iguazu, Misiones, Argentina.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Bahia. Salvador, BA, Brasil.
University of Texas Medical Branch. Galveston, TX, USA.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Emory University. Atlanta, GA, USA.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Biología Subtropical. Nodo Iguazú. Puerto Iguazu, Misiones, Argentina.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Bahia. Salvador, BA, Brasil.
University of Texas Medical Branch. Galveston, TX, USA.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Emory University. Atlanta, GA, USA.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil.
Abstract
To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness
surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous
CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and
paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days postonset
of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases,
and on negative patients.
Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for
convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for
acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were
88.5% and 83.9%, respectively.
Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun
test returned more equivocal results and presented a slightly lower specificity, which might result in a
higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of
CHIKV infection is lower.
Keywords in Portuguese
PrecisãoSensibilidade e especificidade
Desempenho de diagnóstico
Vírus Chikungunya
Ensaio de imunoabsorção enzimática
ELISA
Keywords
AccuracySensitivity and specificity
Diagnostic performance
Chikungunya virus
Enzyme-linked immunosorbent assay
ELISA
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