Author | Rodrigues, Ana Carolina Borges da Cruz | |
Author | Oliveira, Felipe P. de | |
Author | Dias, Rosane Borges | |
Author | Sales, Caroline Brandi Schlaepfer | |
Author | Rocha, Clarissa Araújo Gurgel | |
Author | Soares, Milena Botelho Pereira | |
Author | Costa, Emmanoel Vilaça | |
Author | Silva, Felipe Moura Araujo da | |
Author | Gomes, Waldireny Rocha | |
Author | Koolen, Hector Henrique Ferreira | |
Author | Bezerra, Daniel Pereira | |
Access date | 2019-02-12T17:15:38Z | |
Available date | 2019-02-12T17:15:38Z | |
Document date | 2019 | |
Citation | RODRIGUES, Ana Carolina Borges da Cruz et al. In vitro and in vivo anti-leukemia activity of the stem bark of Salacia impressifolia (Miers) A. C. Smith (Celastraceae). Journal of Ethnopharmacology, v. 231, p. 516–524, 2019. | pt_BR |
ISSN | 0378-8741 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/31583 | |
Sponsorship | Brazilian agencies Coordenação de Aperfeiçoamento de
Pessoal de Nível Superior (CAPES), Conselho Nacional de
Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de
Amparo à Pesquisa do Estado da Bahia (FAPESB) | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Salacia impressionifolia | pt_BR |
Subject in Portuguese | Celastraceae | pt_BR |
Subject in Portuguese | Anti-leucemia | pt_BR |
Subject in Portuguese | HL-60 | pt_BR |
Title | In vitro and in vivo anti-leukemia activity of the stem bark of Salacia impressifolia (Miers) A. C. Smith (Celastraceae) | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.jep.2018.11.008 | |
Abstract | Salacia impressifolia (Miers) A. C. Smith (family Celastraceae) is a traditional medicinal plant found in the Amazon Rainforest known as "miraruíra", "cipó-miraruíra" or "panu" and is traditionally used to treat dengue, flu, inflammation, pain, diabetes, male impotency, renal affections, rheumatism and cancer. Aim of the study: The aim of this study was to investigate in vitro and in vivo anti-leukemia activity of
the stem bark of S. impressifolia in experimental models. Materials and methods: The in vitro cytotoxic activity of
extracts, fractions and quinonemethide triterpenes (22-hydroxytingenone, tingenone and pristimerin) from the
stem bark of S. impressifolia in cultured cancer cells was determined. The in vivo antitumor activity of the ethyl
acetate extract (EAE) and of its fraction (FEAE.3) from the stem bark of S. impressifolia was assessed in C.B-17
severe combined immunodeficient (SCID) mice engrafted with human promyelocytic leukemia HL-60 cells.
Results: The extract EAE, its fraction FEAE.3, and quinonemethide triterpenes exhibited potent cytotoxicity
against cancer cell lines, including in vitro anti-leukemia activity against HL-60 and K-562 cells. Moreover,
extract EAE and its fraction FEAE.3 inhibited the in vivo development of HL-60 cells engrafted in C.B-17 SCID
mice. Tumor mass inhibition rates were measured as 40.4% and 81.5% for the extract EAE (20 mg/kg) and for its
fraction FEAE.3 (20 mg/kg), respectively. Conclusions: Ethyl acetate extract and its fraction from the stem bark of
S. impressifolia exhibit in vitro and in vivo anti-leukemia activity that can be attributed to their quinonemethide
triterpenes. These data confirm the ethnopharmacological use of this species and may contribute to the development
of a novel anticancer herbal medicine. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Federal University of Bahia. Institute of Health Sciences. Department of Biomorphology. Salvador, BA, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Center of Biotechnology and Cell Therapy. Salvador, BA, Brazil. | pt_BR |
Affilliation | Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil. | pt_BR |
Affilliation | Federal University of Amazonas. Department of Chemistry. Manaus, AM, Brazil. | pt_BR |
Affilliation | Federal University of Amazonas. Health and Biotechnology Institute. Coari, AM, Brazil. | pt_BR |
Affilliation | Amazonas State University. Metabolomics and Mass Spectrometry Research Group. Manaus, AM, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Subject | Salacia impressifolia | pt_BR |
Subject | Celastraceae | pt_BR |
Subject | Anti-leukemia | pt_BR |
Subject | HL-60 | pt_BR |