Author | Monteiro, Joana Paixão | |
Author | Ferraro, Geraldo Argolo | |
Author | Oliveira, Tulio de | |
Author | Goldani, Luciano Z | |
Author | Kashima, Simone G | |
Author | Alcantara, Luiz Carlos Junior | |
Author | Morgado, Mariza Gonçalves | |
Author | Bou-Habib, Dumith Chequer | |
Author | Castro Filho, Bernardo Galvão | |
Access date | 2011-08-21T01:32:46Z | |
Available date | 2011-08-21T01:32:46Z | |
Document date | 2007 | |
Citation | MONTEIRO, J. P. Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil. AIDS Research and Human Retroviruses, v. 23, n. 1, p. 135-143, 2007. | pt_BR |
ISSN | 0889-2229 | |
URI | https://www.arca.fiocruz.br/handle/icict/2808 | |
Sponsorship | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), the Brazilian Ministry of Health STD/AIDS Program, CNPq, and FIOCRUZ | pt_BR |
Language | eng | pt_BR |
Rights | open access | |
Title | Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil | pt_BR |
Type | Article | |
DOI | 10.1089/aid.2006.0064 | |
Abstract | The molecular and biological properties of HIV-1 subtype C strains from South Brazil were investigated. We
sequenced gag and env fragments of viruses from 22 HIV-1-infected individuals from Porto Alegre City, which
has the highest frequency of subtype C in the country. The sequences were then compared with other subtype
B, C, and F strains isolated in Brazil and other countries using phylogenetic methods. Amino acid signatures
were identified and correlated with phenotypic characteristics. We identified six strains with subtype
C (27.3%), eight subtype B (36.4%), one subtype F (4.5%), six C/B recombinants (27.3%), and one B/F recombinant
(4.5%). The Brazilian subtype C sequences formed a unique phylogenetic group and presented 6
and 18 specific amino acid signatures in gag and env, respectively. Three distinct patterns of C/B recombinants
presented characteristic Brazilian amino acid substitutions. Subtype C viruses were predominantly R5
and non-syncytium-inducing, while C/B recombinants were R5/X4 and syncytium-inducing viruses. These
findings suggest that subtype C viruses circulating in Brazil are the result of a unique introduction into the
country. Recombination events between subtypes B and C have been occurring frequently for more than 10
years in South Brazil. Biological characterization confirms the hypothesis that subtype C is distinct from the
others in the evolution of coreceptor utilization. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | University of Oxford. Department of Zoology. Oxford, UK | pt_BR |
Affilliation | Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Porto Alegre, RS, Brasil | pt_BR |
Affilliation | Universidade de São Paulo. Hemocentro de Ribeirão Preto. Ribeirão Preto, SP, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA. Brasil | pt_BR |
DeCS | Infecções por HIV | pt_BR |
DeCS | HIV-1 | pt_BR |
DeCS | Recombinação Genética | pt_BR |
DeCS | DNA Viral | pt_BR |
DeCS | Reação em Cadeia da Polimerase | pt_BR |
DeCS | Análise de Sequência de DNA | pt_BR |