Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/2808
Title: Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil
Authors: Monteiro, Joana Paixão
Ferraro, Geraldo Argolo
Oliveira, Tulio de
Goldani, Luciano Z
Kashima, Simone G
Alcantara, Luiz Carlos Júnior
Morgado, Mariza Gonçalves
Bou-Habib, Dumith Chequer
Castro Filho, Bernardo Galvão
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. Brasil
University of Oxford. Department of Zoology, Oxford, UK
Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre, Porto Alegre, RS. Brasil
Universidade de São Paulo. Hemocentro de Ribeirão Preto, Ribeirão Preto, São Paulo, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. Brasil
Fundação Oswaldo Cruz. Instituto oswaldo Cruz, Rio de Janeiro, RJ. Brasil
Fundação Oswaldo Cruz. Instituto oswaldo Cruz, Rio de Janeiro, RJ. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA. Brasil
Abstract: The molecular and biological properties of HIV-1 subtype C strains from South Brazil were investigated. We sequenced gag and env fragments of viruses from 22 HIV-1-infected individuals from Porto Alegre City, which has the highest frequency of subtype C in the country. The sequences were then compared with other subtype B, C, and F strains isolated in Brazil and other countries using phylogenetic methods. Amino acid signatures were identified and correlated with phenotypic characteristics. We identified six strains with subtype C (27.3%), eight subtype B (36.4%), one subtype F (4.5%), six C/B recombinants (27.3%), and one B/F recombinant (4.5%). The Brazilian subtype C sequences formed a unique phylogenetic group and presented 6 and 18 specific amino acid signatures in gag and env, respectively. Three distinct patterns of C/B recombinants presented characteristic Brazilian amino acid substitutions. Subtype C viruses were predominantly R5 and non-syncytium-inducing, while C/B recombinants were R5/X4 and syncytium-inducing viruses. These findings suggest that subtype C viruses circulating in Brazil are the result of a unique introduction into the country. Recombination events between subtypes B and C have been occurring frequently for more than 10 years in South Brazil. Biological characterization confirms the hypothesis that subtype C is distinct from the others in the evolution of coreceptor utilization.
DeCS: Infecções por HIV
HIV-1
Recombinação Genética
DNA Viral
Reação em Cadeia da Polimerase
Análise de Sequência de DNA
Issue Date: 2007
Citation: MONTEIRO, J. P. Genetic and biologic characterization of HIV type 1 subtype C isolates from south Brazil. AIDS Research and Human Retroviruses, v. 23, n. 1, p. 135-143, 2007.
DOI: 10.1089/aid.2006.0064
ISSN: 0889-2229
Appears in Collections:IGM - Artigos de Periódicos

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