Author | Croda, Julio Henrique Rosa | |
Author | Ramos, João G. R | |
Author | Matsunaga, James | |
Author | Queiroz, Adriano | |
Author | Homma, Akira | |
Author | Riley, Lee Woodland | |
Author | Haake, David A | |
Author | Reis, Mitermayer Galvão dos | |
Author | Ko, Albert Icksang | |
Access date | 2011-08-15T18:03:05Z | |
Available date | 2011-08-15T18:03:05Z | |
Document date | 2007 | |
Citation | CRODA, J. et al. Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis.Journal of Clinical Microbiology, v.45, n.5, p.1528-1534, 2007. | pt_BR |
ISSN | 0095-1137 | |
URI | https://www.arca.fiocruz.br/handle/icict/2796 | |
Sponsorship | Este trabalho foi financiado por concessões do Ministério da Saúde, Fundação Oswaldo Cruz, BioManguinhos e pelo CNPq e FINEP, fundos de pesquisa médica e institutos nacionais de saúde. | pt_BR |
Language | eng | pt_BR |
Publisher | American Society for Microbiology | pt_BR |
Rights | open access | en |
Subject in Portuguese | Leptospirose aguda | pt_BR |
Subject in Portuguese | Imunoglobulina Leptospira | pt_BR |
Subject in Portuguese | Diagnóstico Sorológico | pt_BR |
Title | Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1128/JCM.02344-06 | |
Abstract | There is an urgent need for improved diagnosis of leptospirosis, an emerging infectious disease which imparts a large disease burden in developing countries. We evaluated the use of Leptospira immunoglobulin (Ig)-like (Lig) proteins as a serodiagnostic marker for leptospirosis. Lig proteins have bacterial immunoglobulin-
like (Big) tandem repeat domains, a moiety found in virulence factors in other pathogens. Sera from patients identified during urban outbreaks in Brazil reacted strongly with immunoblots of a recombinant
fragment comprised of the second to sixth Big domains of LigB from L. interrogans serovar Copenhageni, the principal agent for transmission in this setting. Furthermore, the sera recognized an analogous LigB fragment derived from L. kirschneri serovar Grippotyphosa, a pathogenic serovar which is not endemic to the study area. The immunoblot assay detected anti-LigB IgM antibodies in sera from 92% (95% confidence interval, 85 to
96%) of patients during acute-phase leptospirosis. The assay had a sensitivity of 81% for sera from patients with less than 7 days of illness. Anti-LigB antibodies were found in sera from 57% of the patients who did not have detectable anti-whole-Leptospira responses as detected by IgM enzyme-linked immunosorbent assay and microagglutination test. The specificities of the assay were 93 to 100% and 90 to 97% among sera from healthy
individuals and patients with diseases that have clinical presentations that overlap with those of leptospirosis,
respectively. These findings indicate that the antibody response to this putative virulence determinant is a
sensitive and specific marker for acute infection. The use of this marker may aid the prompt and timely diagnosis required to reduce the high mortality associated with severe forms of the disease. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Veterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California, USA / UCLA School of Medicine. Department of Medicine. Los Angeles, California, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | University of California. School of Public Health. Berkeley, California, USA | pt_BR |
Affilliation | Veterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California, USA / UCLA School of Medicine. Department of Medicine. Los Angeles, California, USA | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. Ithaca, New York, USA | pt_BR |
Subject | Acute leptospirosis | pt_BR |
Subject | Leptospira immunoglobulin | pt_BR |
Subject | Serological Diagnosis | pt_BR |
DeCS | Anticorpos Antibacterianos | pt_BR |
DeCS | Proteínas de Bactérias | pt_BR |
DeCS | Marcadores Biológicos | pt_BR |
DeCS | Brasil | pt_BR |
DeCS | Estudos de Casos e Controles | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Imunoglobulina G | pt_BR |
DeCS | Imunoglobulina M | pt_BR |
DeCS | Imunoglobulinas | pt_BR |
DeCS | Leptospira | pt_BR |
DeCS | Leptospirose | pt_BR |