Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/2796
Title: Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis
Authors: Croda, Julio Henrique Rosa
Ramos, João G. R
Matsunaga, James
Queiroz, Adriano
Homma, Akira
Riley, Lee Woodland
Haake, David A
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Veterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California, USA / UCLA School of Medicine. Department of Medicine. Los Angeles, California, USA
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, Brasil
University of California. School of Public Health. Berkeley, California, USA
Veterans Affairs Greater Los Angeles Healthcare System. Division of Infectious Diseases. Los Angeles, California, USA / UCLA School of Medicine. Department of Medicine. Los Angeles, California, USA
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. Ithaca, New York, USA
Abstract: There is an urgent need for improved diagnosis of leptospirosis, an emerging infectious disease which imparts a large disease burden in developing countries. We evaluated the use of Leptospira immunoglobulin (Ig)-like (Lig) proteins as a serodiagnostic marker for leptospirosis. Lig proteins have bacterial immunoglobulin- like (Big) tandem repeat domains, a moiety found in virulence factors in other pathogens. Sera from patients identified during urban outbreaks in Brazil reacted strongly with immunoblots of a recombinant fragment comprised of the second to sixth Big domains of LigB from L. interrogans serovar Copenhageni, the principal agent for transmission in this setting. Furthermore, the sera recognized an analogous LigB fragment derived from L. kirschneri serovar Grippotyphosa, a pathogenic serovar which is not endemic to the study area. The immunoblot assay detected anti-LigB IgM antibodies in sera from 92% (95% confidence interval, 85 to 96%) of patients during acute-phase leptospirosis. The assay had a sensitivity of 81% for sera from patients with less than 7 days of illness. Anti-LigB antibodies were found in sera from 57% of the patients who did not have detectable anti-whole-Leptospira responses as detected by IgM enzyme-linked immunosorbent assay and microagglutination test. The specificities of the assay were 93 to 100% and 90 to 97% among sera from healthy individuals and patients with diseases that have clinical presentations that overlap with those of leptospirosis, respectively. These findings indicate that the antibody response to this putative virulence determinant is a sensitive and specific marker for acute infection. The use of this marker may aid the prompt and timely diagnosis required to reduce the high mortality associated with severe forms of the disease.
Keywords: Acute leptospirosis
Leptospira immunoglobulin
Serological Diagnosis
keywords: Leptospirose aguda
Imunoglobulina Leptospira
Diagnóstico Sorológico
DeCS: Anticorpos Antibacterianos
Proteínas de Bactérias
Marcadores Biológicos
Brasil
Estudos de Casos e Controles
Humanos
Imunoglobulina G
Imunoglobulina M
Imunoglobulinas
Leptospira
Leptospirose
Issue Date: 2007
Publisher: American Society for Microbiology
Citation: CRODA, J. et al. Leptospira immunoglobulin-like proteins as a serodiagnostic marker for acute leptospirosis.Journal of Clinical Microbiology, v.45, n.5, p.1528-1534, 2007.
DOI: 10.1128/JCM.02344-06
ISSN: 0095-1137
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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