Author | Barral Netto, Manoel | |
Author | Schriefer, Albert | |
Author | Vinhas, Vera | |
Author | Almeida, A. R | |
Access date | 2018-05-09T19:14:21Z | |
Available date | 2018-05-09T19:14:21Z | |
Document date | 1990 | |
Citation | BARRAL NETTO, M. et al. Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom. Toxicon, v . 28, n. 9, p. 1053-1061, 1990. | pt_BR |
ISSN | 0041-0101 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/26349 | |
Description | Manoel, Barral Netto. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. | pt_BR |
Sponsorship | CNPq's (Conselho National de Desenvolvimento Cientifico e Tecnologico) scientific initiation fellowship . This project was supported by Financiadora de Estudos e Projetos (FINEP). | pt_BR |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Bothrops jararaca | pt_BR |
Subject in Portuguese | Veneno | pt_BR |
Subject in Portuguese | Venenos de Cascavel | pt_BR |
Subject in Portuguese | Ensaio imunoenzimático | pt_BR |
Subject in Portuguese | Soro | pt_BR |
Title | Enzyme-linked immunosorbent assay for the detection of Bothrops Jararaca Venom | pt_BR |
Type | Article | pt_BR |
Abstract | Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca
venom. Toxicon 28, 1053-1061, 1990.-This study reports an enzyme-linked
immunosorbent assay for detecting Bothrops jararaca venom in fluids,
employing the sandwich method with biotin/avidin amplification. The assay
exhibits high accuracy in correlating optical densities with venom concentrations
(r = 0.98), high reproducibility, low background and limited crossreactivity
with venom from other snake genera . Nevertheless, it was unable to
distinguish among venoms from different bothropic species. Using this method
we evaluated the serum kinetics of Bothrops jararaca venom in C57BL/6 mice.
High concentrations were found in serum just 15 min after injection
(151 f41 ng/ml; mean±S.D.), followed by a progressive fall (102 f46, 74 f39
and 50±22ng/ml after 1, 3 and 6 hr respectively), being undetectable by 24 hr.
Such serum kinetics indicates a pattern of a rapid absorption of venom from
the inoculation site, followed by a slow and progressive drop in its serum
levels . This ELISA was a reliable tool in the determination of Bothrops
jararaca venom levels in mouse serum, and may become useful in other fields
of bothropic venom research | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
Subject | Bothrops jararaca | pt_BR |
Subject | Venom | pt_BR |
Subject | Jararaca venom | pt_BR |
Subject | Immunosorbent assay | pt_BR |
Subject | Serum | pt_BR |