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https://www.arca.fiocruz.br/handle/icict/26349
ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF BOTHROPS JARARACA VENOM
Affilliation
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Abstract
Enzyme-linked immunosorbent assay for the detection of Bothrops jararaca
venom. Toxicon 28, 1053-1061, 1990.-This study reports an enzyme-linked
immunosorbent assay for detecting Bothrops jararaca venom in fluids,
employing the sandwich method with biotin/avidin amplification. The assay
exhibits high accuracy in correlating optical densities with venom concentrations
(r = 0.98), high reproducibility, low background and limited crossreactivity
with venom from other snake genera . Nevertheless, it was unable to
distinguish among venoms from different bothropic species. Using this method
we evaluated the serum kinetics of Bothrops jararaca venom in C57BL/6 mice.
High concentrations were found in serum just 15 min after injection
(151 f41 ng/ml; mean±S.D.), followed by a progressive fall (102 f46, 74 f39
and 50±22ng/ml after 1, 3 and 6 hr respectively), being undetectable by 24 hr.
Such serum kinetics indicates a pattern of a rapid absorption of venom from
the inoculation site, followed by a slow and progressive drop in its serum
levels . This ELISA was a reliable tool in the determination of Bothrops
jararaca venom levels in mouse serum, and may become useful in other fields
of bothropic venom research
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