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https://www.arca.fiocruz.br/handle/icict/20777
EFFECTS OF THE ETHANOL EXTRACT OF THE INNER BARK OF SYDEROXYLUM OBTUSIFOLIUM IN THE CYCLOPHOSPHAMIDE-INDUCED CYSTITIS IN RATS
Cyclophosphamide
Inflammation
Lipid peroxidation
Hemorrhagic cystitis
Author
Affilliation
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Federal University of Sergipe. Department of Physiology. São Cristóvão, SE, Brazil
Abstract
In this study, the ethanol extract of the inner bark of the Syderoxylum obtusifolium (EESob) was tested
in the model of cyclophosphamide-induced hemorrhagic cystitis. Male Wistar rats were submitted to
the injection of cyclophosphamide, 1 h after the treatment with EESob (200 or 400 mg/kg) or vehicle.
After 24 h, the urinary bladder was excised and the edema, myeloperoxidase activity and
malondialdehyde formation were measured. Also, myeloperoxidase activity and malondialdehyde
formation in lung, spleen and liver and leukocyte counts in the peripheral blood were evaluated.
Injection of cyclophosphamide increased myeloperoxidase activity and edema. The former was
inhibited by the EESob at 400 mg/kg, but the latter was not affected. Although cyclophosphamide did
not alter the bladder malondialdehyde formation, pre-treatment with EESob at both doses markedly
increased this parameter. Lung and liver parameters or leukocyte counts were not altered by EESob.
The spleen myeloperoxidase activity and malondialdehyde formation were not affected by
cyclophosphamide, but were increased by the treatment with EESob. These results suggests that
EESob administration to rats decrease myeloperoxidase activity in bladder tissue, but is accompanied
by lipoperoxidation in this tissue, as well as in spleen, which do not support the use of EESob to treat
cystitis.
Keywords
Syderoxylum obtusifoliumCyclophosphamide
Inflammation
Lipid peroxidation
Hemorrhagic cystitis
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