Author | Cassemiro, Klécia M. S. M. | |
Author | Burlandy, Fernanda M. | |
Author | Silva, Edson E. da | |
Access date | 2017-04-20T14:08:50Z | |
Available date | 2017-04-20T14:08:50Z | |
Document date | 2016 | |
Citation | CASSEMIRO, Klécia M. S. M.; BURLANDY, Fernanda M.; SILVA, Edson E,. da. Rare natural type 3/type 2 intertypic capsid recombinant vaccine-related poliovirus isolated from a case of acute flaccid paralysis in Brazil, 2015. Journal of General Virology, v.97, p.1545-1550, 2016. | pt_BR |
ISSN | 0022-1317 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/18593 | |
Language | eng | pt_BR |
Publisher | Microbiology Society | pt_BR |
Rights | restricted access | |
Subject in Portuguese | Poliovirus | pt_BR |
Subject in Portuguese | Paralisia flácida aguda | pt_BR |
Subject in Portuguese | Administração oral da vacina contra o poliovírus | pt_BR |
Subject in Portuguese | Brasil | pt_BR |
Title | Rare natural type 3/type 2 intertypic capsid recombinant vaccine-related poliovirus isolated from a case of acute flaccid paralysis in Brazil, 2015 | pt_BR |
Type | Article | |
DOI | 10.1099/jgv.0.000484 | |
Abstract | A natural type 3/type 2 intertypic capsid recombinant vaccine-related poliovirus was isolated from an acute flaccid paralytic case in Brazil. Genome sequencing revealed the uncommon location of the crossover site in the VP1 coding region (nucleotides 3251-3258 of Sabin 3 genome). The Sabin 2 donor sequence replaced the last 118 nt of VP1, resulting in the substitution of the complete antigenic site IIIa by PV2-specific amino acids. The low overall number of nucleotide substitutions in P1 region indicated that the predicted replication time of the isolate was about 8-9 weeks. Two of the principal determinants of attenuation in Sabin 3 genomes were mutated (U472C and C2493U), but the temperature-sensitive phenotype of the isolate was preserved. Our results support the theory that there exists a PV3/PV2 recombination hotspot site in the tail region of the VP1 capsid protein and that the recombination may occur soon after oral poliovirus vaccine administration. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil. | pt_BR |
Subject | poliovirus | pt_BR |
Subject | acute flaccid paralysis | pt_BR |
Subject | oral poliovirus vaccine administration | pt_BR |
Subject | Brazil | pt_BR |
Subject | PV3/PV2 recombination hotspot site | pt_BR |
Subject | VP1 capsid protein | pt_BR |
e-ISSN | 1465-2099 | |
Embargo date | 2030-01-01 | |