Please use this identifier to cite or link to this item: http://www.arca.fiocruz.br/handle/icict/18593
Title: Rare natural type 3/type 2 intertypic capsid recombinant vaccine-related poliovirus isolated from a case of acute flaccid paralysis in Brazil, 2015
Authors: Cassemiro, Klécia M. S. M.
Burlandy, Fernanda M.
Silva, Edson E. da
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ. Brasil.
Abstract: A natural type 3/type 2 intertypic capsid recombinant vaccine-related poliovirus was isolated from an acute flaccid paralytic case in Brazil. Genome sequencing revealed the uncommon location of the crossover site in the VP1 coding region (nucleotides 3251-3258 of Sabin 3 genome). The Sabin 2 donor sequence replaced the last 118 nt of VP1, resulting in the substitution of the complete antigenic site IIIa by PV2-specific amino acids. The low overall number of nucleotide substitutions in P1 region indicated that the predicted replication time of the isolate was about 8-9 weeks. Two of the principal determinants of attenuation in Sabin 3 genomes were mutated (U472C and C2493U), but the temperature-sensitive phenotype of the isolate was preserved. Our results support the theory that there exists a PV3/PV2 recombination hotspot site in the tail region of the VP1 capsid protein and that the recombination may occur soon after oral poliovirus vaccine administration.
Keywords: poliovirus
acute flaccid paralysis
oral poliovirus vaccine administration
Brazil
PV3/PV2 recombination hotspot site
VP1 capsid protein
keywords: Poliovirus
Paralisia flácida aguda
Administração oral da vacina contra o poliovírus
Brasil
Issue Date: 2016
Publisher: Microbiology Society
Citation: CASSEMIRO, Klécia M. S. M.; BURLANDY, Fernanda M.; SILVA, Edson E,. da. Rare natural type 3/type 2 intertypic capsid recombinant vaccine-related poliovirus isolated from a case of acute flaccid paralysis in Brazil, 2015. Journal of General Virology, v.97, p.1545-1550, 2016.
DOI: 10.1099/jgv.0.000484
ISSN: 0022-1317
Copyright: restricted access
Appears in Collections:IOC - Artigos de Periódicos

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