Author | Fernandez, José C. Couto | |
Author | Janssens, Wouter | |
Author | Heyndrickx, Leo | |
Author | Motte, Jan | |
Author | Fransen, Katrien | |
Author | Peeters, Martine | |
Author | Delaporte, Eric | |
Author | Castro Filho, Bernardo Galvão | |
Author | Piot, Peter | |
Author | van der Groen, Guido | |
Access date | 2016-07-15T16:53:26Z | |
Available date | 2016-07-15T16:53:26Z | |
Document date | 1994 | |
Citation | FERNANDEZ, J. C. C. et al. Genetic and antigenic variability of HIV type 1 in Brazil. AIDS Research and Human Retroviruses, v. 10, n. 9, p. 1157-1163, 1994. | pt_BR |
ISSN | 0889-2229 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/14933 | |
Language | eng | pt_BR |
Publisher | Mary Ann Liebert | pt_BR |
Rights | open access | pt_BR |
Title | Genetic and antigenic variability of HIV type 1 in Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1089/aid.1994.10.1157 | |
Abstract | Six Brazilian strains of human immunodeficiency virus type 1 (HIV-1) were isolated from infected individuals residing in different regions of Brazil between 1987 and 1989. Phylogenetic analysis based on an 860-base pair env fragment, including V3, V4, V5, and the beginning of gp41, classified the Brazilian strains significantly in genotype B, with interhost distances between 5.9 and 13.1% (mean value, 10%). Amino acid sequence analysis of the V3 loop revealed that three strains contained the North American/European GPGR motif as the tip of the loop whereas in the other three strains proline (P) was substituted by tryptophan (W), methionine (M), or phenylalanine (F). A consensus peptide, Bra-cons, was designed containing GWGR as the tip of the loop. Serological reactivity to the Bra-cons peptide and other V3 peptides (MN, SF2, HBX2, RF, MAL, ELI, Z6, and a Côte d'Ivoire peptide, CI-cons) was compared for 114 HIV-1-positive sera from Rio de Janeiro. Sixty-nine sera (60.5%) reacted with peptides belonging to genotype B, of which 10 sera also reacted with peptides belonging to genotype A (n = 7) and D (n = 3). Eighteen sera (15.8%) had binding antibodies to the Bra-cons peptide. A high number of sera (n = 43; 37.7%) had no antibodies to any of the V3 peptides tested. This result suggests that HIV-1 variants with aberrant V3 loops may circulate in Rio de Janeiro. | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium | pt_BR |
Affilliation | Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium | pt_BR |
Affilliation | Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium | pt_BR |
Affilliation | Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium | pt_BR |
Affilliation | Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium | pt_BR |
Affilliation | INSERM U13. IMEA-Hôpital Bichat-Claude Bernard. Paris, France | pt_BR |
Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
Affilliation | Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium | pt_BR |
Affilliation | Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium | pt_BR |
Subject | HIV-1 | pt_BR |
Subject | Antigenic Variation | pt_BR |
Subject | Phylogeny | pt_BR |
Subject | HIV Antibodies | pt_BR |
Subject | Genotype | pt_BR |
Subject | HIV Infections | pt_BR |
Subject | Brazil | pt_BR |