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https://www.arca.fiocruz.br/handle/icict/14933
GENETIC AND ANTIGENIC VARIABILITY OF HIV TYPE 1 IN BRAZIL
Author
Affilliation
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
INSERM U13. IMEA-Hôpital Bichat-Claude Bernard. Paris, France
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
INSERM U13. IMEA-Hôpital Bichat-Claude Bernard. Paris, France
Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Institute of Tropical Medicine. Division of Microbiology. Department of Infection and Immunity. Antwerp, Belgium
Abstract
Six Brazilian strains of human immunodeficiency virus type 1 (HIV-1) were isolated from infected individuals residing in different regions of Brazil between 1987 and 1989. Phylogenetic analysis based on an 860-base pair env fragment, including V3, V4, V5, and the beginning of gp41, classified the Brazilian strains significantly in genotype B, with interhost distances between 5.9 and 13.1% (mean value, 10%). Amino acid sequence analysis of the V3 loop revealed that three strains contained the North American/European GPGR motif as the tip of the loop whereas in the other three strains proline (P) was substituted by tryptophan (W), methionine (M), or phenylalanine (F). A consensus peptide, Bra-cons, was designed containing GWGR as the tip of the loop. Serological reactivity to the Bra-cons peptide and other V3 peptides (MN, SF2, HBX2, RF, MAL, ELI, Z6, and a Côte d'Ivoire peptide, CI-cons) was compared for 114 HIV-1-positive sera from Rio de Janeiro. Sixty-nine sera (60.5%) reacted with peptides belonging to genotype B, of which 10 sera also reacted with peptides belonging to genotype A (n = 7) and D (n = 3). Eighteen sera (15.8%) had binding antibodies to the Bra-cons peptide. A high number of sera (n = 43; 37.7%) had no antibodies to any of the V3 peptides tested. This result suggests that HIV-1 variants with aberrant V3 loops may circulate in Rio de Janeiro.
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