Author | Queiroz, Mayara Ribeiro de | |
Author | Mamede, Carla Cristine N. | |
Author | Morais, Nadia Cristina G. de | |
Author | Fonseca, Kelly Cortes | |
Author | Sousa, Bruna Barbosa de | |
Author | Migliorini, Thaís M. | |
Author | Pereira, Déborah Fernanda C. | |
Author | Stanziola, Leonilda | |
Author | Calderon, Leonardo A. | |
Author | Silva, Rodrigo Simões | |
Author | Soares, Andreimar Martins | |
Author | Oliveira, Fábio de | |
Access date | 2016-04-19T13:40:24Z | |
Available date | 2016-04-19T13:40:24Z | |
Document date | 2014 | |
Citation | CALDERON, L. A. et al. Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation. BioMed Research International, v. 2014, p. 1-7, 2014. | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/13861 | |
Language | eng | pt_BR |
Publisher | BioMed Research International | pt_BR |
Rights | open access | pt_BR |
Title | Purification and Characterization of BmooAi: A New Toxin from Bothrops moojeni Snake Venom That Inhibits Platelet Aggregation | pt_BR |
Type | Article | pt_BR |
DOI | http://dx.doi.org/10.1155/2014/920942 | |
Abstract | In this paper, we describe the purification/characterization of BmooAi, a new toxin from Bothrops moojeni that inhibits platelet aggregation. The purification of BmooAi was carried out through three chromatographic steps (ion-exchange on a DEAE-Sephacel column, molecular exclusion on a Sephadex G-75 column, and reverse-phase HPLC chromatography on a C2/C18 column).
BmooAi was homogeneous by SDS-PAGE and shown to be a single-chain protein of 15,000 Da. BmooAi was analysed by
MALDI-TOF Spectrometry and revealed two major components with molecular masses 7824.4 and 7409.2 as well as a trace of
protein with a molecular mass of 15,237.4 Da. Sequencing of BmooAi by Edman degradation showed two amino acid sequences: IRDFDPLTNAPENTA and ETEEGAEEGTQ, which revealed no homology to any known toxin from snake venom. BmooAi
showed a rather specific inhibitory effect on platelet aggregation induced by collagen, adenosine diphosphate, or epinephrine in human platelet-rich plasma in a dose-dependent manner, whereas it had little or no effect on platelet aggregation induced by ristocetin. The effect on platelet aggregation induced by BmooAi remained active even when heated to 100∘C. BmooAi could be of medical interest as a new tool for the development of novel therapeutic agents for the prevention and treatment of thrombotic disorders. | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil / Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil./ Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Uberlândia. Instituto de Genética e Bioquímica. Uberlândia, MG, Brazil. | pt_BR |
Affilliation | Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil / Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde e Núcleo de Saúde. Porto Velho, RO, Brazil / Universidade Federal de Rondônia. Porto Velho, RO, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde e Núcleo de Saúde. Porto Velho, RO, Brazil / Universidade Federal de Rondônia. Porto Velho, RO, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde e Núcleo de Saúde. Porto Velho, RO, Brazil / Universidade Federal de Rondônia. Porto Velho, RO, Brazil. | pt_BR |
Affilliation | Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica. Belo Horizonte, MG, Brazil / Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Uberlândia, MG, Brazil. | pt_BR |
Subject | BmooAi | en |
Subject | New Toxin | en |
Subject | Bothrops moojeni | en |
Subject | Snake Venom | en |
Subject | Platelet Aggregation | en |