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IMMUNIZATION WITH CYTOPLASMIC REPETITIVE ANTIGEN AND FLAGELLAR REPETITIVE ANTIGEN OF TRYPANOSOMA CRUZI STIMULATES A CELLULAR IMMUNE RESPONSE IN MICE
Imunidade Celular
Proteínas de Protozoários/imunologia
Trypanosoma cruzi/imunologia
Animais
Antígenos de Protozoários/administração & dosagem
Citocinas/biossíntese
Citometria de Fluxo
Imunidade Celular/imunologia
Imunização
Ativação Linfocitária/imunologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
Author
Affilliation
Abstract
In previous studies, we demonstrated that CRA and FRA recombinant proteins, used for diagnosis of Chagas' disease, elicited a humoral immune response in susceptible and resistant mice. To understand better the immune response to these proteins, we have evaluated, the cellular immune response in CRA- and in FRA-immunized BALB/c and C57BL/6 mice. A specific cellular lymphoproliferative response was observed in both strains of mice. Spleen cell cultures mainly from CRA-immunized C57BL/6 and FRA-immunized BALB/c mice produced high levels of IFN-y, indicating the induction of a Type 1 immune response. Regarding the T cell subsets, CD4+ T cells were the major source of IFN-y in CRA- and FRA-immunized mice. These results suggest that CRA and FRA are important immunogens in inducing a Type 1 immune response and that they may be considered as potential vaccine antigens.
DeCS
Antígenos de Protozoários/imunologiaImunidade Celular
Proteínas de Protozoários/imunologia
Trypanosoma cruzi/imunologia
Animais
Antígenos de Protozoários/administração & dosagem
Citocinas/biossíntese
Citometria de Fluxo
Imunidade Celular/imunologia
Imunização
Ativação Linfocitária/imunologia
Masculino
Camundongos
Camundongos Endogâmicos BALB C
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