The purpose of this work was to apply a simple method for acquisition of power output (PO) during the Wingate Anaerobic Test (WAnT) at a high sampling rate ( S R ) and to compare the effect of lower S R on the measurements extracted from the PO. 26 male subjects underwent 2 WAnTs on a cycle ergometer. The reference PO was calcu-lated at 30 Hz as a function of the linear veloc-ity, the moment of inertia and the frictional load. The PO was sampled at 0.2, 0.5, 1, 2 and 5 Hz. Both the peak (16.03 ± 2.22 W · kg −1 ) and mean PO (10.34 ± 1.01 W · kg −1 ) presented lower rela-tive values when the S R was lower. Peak PO was attenuated by 0.29 – 42.07 % for decreasing sam-pling rates, resulting in different values for 0.2 and 1 Hz ( P< 0.001). When the S R was 0.2 Hz, the time to peak was delayed by 53.81 % ( P< 0.001) and the fatigue index was attenuated by 22.12 % ( P< 0.001). In conclusion, due to the differences achieved here and the fact that the peak flywheel frequency is around 2.3 Hz, we strongly recom-mend that the PO be sampled at 5 Hz instead of 0.2 Hz in order to avoid biased errors and misun-derstandings of the WAnT results.