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DESCRIPTION OF MICROSPORIDIA IN SIMULIDS: MOLECULAR AND MORPHOLOGICAL CHARACTERIZATION OF MICROSPORIDIA IN THE LARVAE OF SIMULIUM PERTINAX KOLLAR (DIPTERA: SIMULIIDAE)
Autor
Afiliación
Instituto Butantan. Laboratório de Parasitologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, Brasil
Superintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Disciplina de Infectologia. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Gastroenterologia. São Paulo, SP, Brasil
Superintendência de Controle de Endemias. Laboratório de Simulídeos, Caraguatatuba, SP, Brasil
United States Department of Agriculture Mosquito. Florida, USA / Fly Research Unit. Gainesville, Florida, USA
Superintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Imunoparasitologia. Salvador, BA, Brasil
Superintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Disciplina de Infectologia. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Departamento de Medicina. Disciplina de Gastroenterologia. São Paulo, SP, Brasil
Superintendência de Controle de Endemias. Laboratório de Simulídeos, Caraguatatuba, SP, Brasil
United States Department of Agriculture Mosquito. Florida, USA / Fly Research Unit. Gainesville, Florida, USA
Superintendência de Controle de Endemias. Laboratório de Entomologia Médica. São Paulo, SP, Brasil
Resumen en ingles
Introduction: Microsporidia constitute the most common black fl y pathogens, although the species’ diversity, seasonal occurrence
and transmission mechanisms remain poorly understood. Infections by this agent are often chronic and non-lethal, but they can
cause reduced fecundity and decreased longevity. The objective of this study was to identify microsporidia infecting Simulium
(Chirostilbia) pertinax (Kollar, 1832) larvae from Caraguatatuba, State of São Paulo, Brazil, by molecular and morphological
characterization. Methods: Larvae were collected at a single point in a stream in a rural area of the city and were kept under
artifi cial aeration until analysis. Polydispyrenia spp. infection was characterized by the presence of at least 32 mononuclear spores
measuring 6.9 ± 1.0 x 5.0 ± 0.7μm in persistent sporophorous vesicles. Similarly, Amblyospora spp. were characterized by the
presence of eight uninucleate spores measuring 4.5 x 3.5μm in sporophorous vesicles. Results: The molecular analysis confi rmed
the presence of microsporidian DNA in the 8 samples (prevalence of 0.51%). Six samples (Brazilian larvae) were related to
Polydispyrenia simulii and Caudospora palustris reference sequences but in separate clusters. One sample was clustered with
Amblyospora spp. Edhazardia aedis was the positive control taxon. Conclusions: Samples identifi ed as Polydispyrenia spp. and
Amblyospora spp. were grouped with P. simulii and Amblyospora spp., respectively, corroborating previous results. However,
the 16S gene tree showed a considerable distance between the black fl y-infecting Amblyospora spp. and the mosquito-infecting
spp. This distance suggests that these two groups are not congeneric. Additional genomic region evaluation is necessary to obtain
a coherent phylogeny for this group.
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