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IMMUNOHISTOCHEMICAL DETECTION OF KI-67 IS NOT ASSOCIATED WITH TUMOR-INFILTRATING MACROPHAGES AND CYCLOOXYGENASE-2 IN ORAL SQUAMOUS CELL CARCINOMA.
Imunohistochemistry
Ki-67
Macrophages
Squamous cell carcinoma
Ciclo-Oxigenase 2/análise
Antígeno Ki-67/análise
Macrófagos/patologia
Neoplasias Bucais/patologia
Antígenos CD/análise
Antígenos de Diferenciação Mielomonocítica/análise
Carcinoma in Situ/enzimologia
Carcinoma de Células Escamosas/enzimologia
Núcleo Celular/ultraestrutura
Corantes/uso diagnóstico
Citoplasma/ultraestrutura
Células Epiteliais/enzimologia
Humanos
Imuno-Histoquímica
Neoplasias Bucais/enzimologia
Invasividade Neoplásica
Marcadores Biológicos de Tumor/análise
Autor
Afiliación
Federal University of Bahia. Institute of Health Sciences. Department of Bio-morphology. Salvador, BA, Brasil / Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Rio de Janeiro. School of Dentistry. Department of Pathology and Oral Diagnosis. Rio de Janeiro, RJ, Brasil
Federal University of Bahia. School of Dentistry. Laboratory of Oral Surgical Pathology. Salvador, BA, Brasil
Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Rio de Janeiro. School of Dentistry. Department of Pathology and Oral Diagnosis. Rio de Janeiro, RJ, Brasil
Federal University of Bahia. School of Dentistry. Laboratory of Oral Surgical Pathology. Salvador, BA, Brasil
Resumen en ingles
BACKGROUND: An inflammatory component consisting of cells and chemical mediators may influence the proliferation and dissemination of the oral squamous cell carcinoma (OSCC). In the present study, we evaluated the possible relationship between Ki-67, tumor-associated macrophages (TAMs), and COX-2 in OSCCs. In addition, the immunodetection of these proteins was associated with different histological grades of malignancy, including invasive and in situ tumors. METHODS: Twenty-seven OSCC cases were examined by light microscopy using criteria adopted WHO, and immunohistochemistry for Ki-67, CD68, and COX-2 using EnVision System in invasive and in situ lesions. Immunohistochemical detection of these proteins was assessed and scored for COX-2, and results were compared with their histological grades of malignancy. RESULTS: A correlation between Ki-67, COX-2, and CD68 was not found. Histological grade of malignancy (HDM) was associated with the Ki-67 immunostaining (P = 0.00), but this was not observed regarding both CD68 (P = 0.51) and COX-2 (P = 0.89). Furthermore, there was a COX-2 overexpression in 62.96% of the sample, and a high density of TAMs in both OSCCs and in situ carcinomas. CONCLUSIONS: Imunolabeling for Ki-67 was directly correlated with less-differentiated tumors, suggesting that this marker may contribute to understand the biological behavior of OSCC, and help to distinguish risk groups of OSCC. Furthermore, the lack of correlation between Ki-67, COX-2, and CD68 indicates that the latter two markers may play a pivotal role in oral carcinogenesis. However, further studies are needed to clarify their contribution for cell proliferation and tumor differentiation.
Palabras clave en ingles
Cyclooxygenase-2Imunohistochemistry
Ki-67
Macrophages
Squamous cell carcinoma
DeCS
Carcinoma de Células Escamosas/patologiaCiclo-Oxigenase 2/análise
Antígeno Ki-67/análise
Macrófagos/patologia
Neoplasias Bucais/patologia
Antígenos CD/análise
Antígenos de Diferenciação Mielomonocítica/análise
Carcinoma in Situ/enzimologia
Carcinoma de Células Escamosas/enzimologia
Núcleo Celular/ultraestrutura
Corantes/uso diagnóstico
Citoplasma/ultraestrutura
Células Epiteliais/enzimologia
Humanos
Imuno-Histoquímica
Neoplasias Bucais/enzimologia
Invasividade Neoplásica
Marcadores Biológicos de Tumor/análise
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