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https://www.arca.fiocruz.br/handle/icict/8386
RELEASE OF CYTOKINES BY STIMULATED PERIPHERAL BLOOD MONONUCLEAR CELLS IN CHRONIC PERIODONTITIS
Citocinas/sangue
Leucócitos Mononucleares/metabolismo
Adulto
Idoso
Células Cultivadas
Escherichia coli
Feminino
Hemorragia Gengival/sangue
Humanos
Mediadores da Inflamação/sangue
Interleucina-10/sangue
Leucócitos Mononucleares/efeitos de drogas
Lipopolissacarídeos/farmacologia
Masculino
Meia-Idade
Perda da Inserção Periodontal/sangue
Bolsa Periodontal/sangue
Hábito de Fumar
Fator de Necrose Tumoral alfa/sangue
Author
Affilliation
UNICAMP. Faculty of Dentistry of Piracicaba. Department of Prosthodontics and Periodontics. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunologia e Parasitologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunologia e Parasitologia. Salvador, BA, Brasil
Guarulhos University. Department of Periodontics, Dental Research Division. Guarulhos, São Paulo, Brasil
University of São Paulo. School of Dentistry. USP. Department of Periodontology. São Paulo, SP, Brasil
University of Manitoba. Faculty of Dentistry. Department of Dental Diagnostic and Surgical Sciences. Division of Periodontics. Bannatyne Ave, Winnipeg, Canada
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunologia e Parasitologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imunologia e Parasitologia. Salvador, BA, Brasil
Guarulhos University. Department of Periodontics, Dental Research Division. Guarulhos, São Paulo, Brasil
University of São Paulo. School of Dentistry. USP. Department of Periodontology. São Paulo, SP, Brasil
University of Manitoba. Faculty of Dentistry. Department of Dental Diagnostic and Surgical Sciences. Division of Periodontics. Bannatyne Ave, Winnipeg, Canada
Abstract
Objective: The emergence of periodontal medicine increased interest in defining the behaviour
of peripheral blood cells in periodontitis subjects in comparison with healthy group.
The aim of this study was to evaluate the levels of interleukin (IL)-8, tumour necrosis factora
(TNF-a), IL-6 and IL-10 released by Escherichia coli lipopolysaccharide (LPS)-stimulated
peripheral blood mononuclear cells (PBMC) obtained from the peripheral blood of chronic
periodontitis subjects.
Design: PBMC samples were isolated from 19 systemically healthy donors, divided into
generalized chronic periodontitis (n = 10) and healthy (n = 9) subjects. Cells were incubated
for 24–48 h in 500 mL wells containing RPMI 1640 and stimulated with 1.0 ng/mL of E. coli LPS.
Supernatants were used to quantify the amounts of IL-8, TNF-a, IL-6 and IL-10 released using
enzyme-linked immunosorbent assay (ELISA).
Results: PBMC cells from periodontitis subjects released higher levels of TNF-a and IL-6 than
those from healthy subjects (P < 0.05). Conversely, the supernatants of the stimulated PBMC
cells obtained from healthy subjects presented higher amounts of IL-8 than those from
periodontitis (P < 0.05). No differences were observed in the levels of IL-10 (P > 0.05) between
groups.
Conclusion: In conclusion, the results of the present study showed that E. coli LPS-stimulated
PBMC from subjects with periodontitis present a different pattern of cytokine release when
compared to PBMC from healthy subjects. This phenomenon could have implications
locally, in periodontitis, as well as in systemic diseases.
DeCS
Periodontite Crônica/sangueCitocinas/sangue
Leucócitos Mononucleares/metabolismo
Adulto
Idoso
Células Cultivadas
Escherichia coli
Feminino
Hemorragia Gengival/sangue
Humanos
Mediadores da Inflamação/sangue
Interleucina-10/sangue
Leucócitos Mononucleares/efeitos de drogas
Lipopolissacarídeos/farmacologia
Masculino
Meia-Idade
Perda da Inserção Periodontal/sangue
Bolsa Periodontal/sangue
Hábito de Fumar
Fator de Necrose Tumoral alfa/sangue
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