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PARASITE-DERIVED TRANS-SIALIDASE BINDS TO HEART TISSUE IN TRYPANOSOMA CRUZI-INFECTED ANIMALS
Miócitos Cardíacos/metabolismo
Neuraminidase/metabolismo
Trypanosoma cruzi/enzimologia
Animais
Anticorpos Antiprotozoários/sangue
Glicoproteínas
Camundongos Endogâmicos BALB C
Camundongos
Camundongos Nus
Miócitos Cardíacos/citologia
Miócitos Cardíacos/parasitologia
Trypanosoma cruzi/imunologia
Virulência
Autor
Afiliación
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Nagasaki University. Institute of Tropical Medicine. Nagasaki, Japan.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
New York University School of Medicine. Department of Medical and Molecular Parasitology. New York, NY, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Nagasaki University. Institute of Tropical Medicine. Nagasaki, Japan.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
New York University School of Medicine. Department of Medical and Molecular Parasitology. New York, NY, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Resumen en portugues
Trypanosoma cruzi is an obligate intracellular protozoan parasite that actively penetrates into non-phagocytic mammalian cells. To accomplish this, the parasite relies on the binding of cell surface ligands. It is reported herein that the T. cruzi trans-sialidase (TS), which is exposed on the parasite surface, binds to mouse heart cells, and should therefore be further studied as a possible cell penetration-related ligand. In addition, as has been proposed elsewhere, the binding of T. cruzi to tissues may turn them into targets for parasite-specific immune reactions. Washed heart sections from T. cruzi-infected mice were subjected to immunoenzymatic staining with antisera against whole T. cruzi and with polyclonal or monoclonal antibodies against TS. The anti-TS antibodies stained both parasites and uninfected heart cells in the vicinity of T. cruzi nest remains/trypomastigotes. On the other hand, an anti-T. cruzi serum, which did not recognize TS, only stained the parasites. In addition, normal heart sections from uninfected nude mice were shown to react with both enzymatically active and inactive recombinant TS molecules, probably through their amino-terminal region, since a recombinant TS lacking this region failed to bind.
DeCS
Miocárdio/metabolismoMiócitos Cardíacos/metabolismo
Neuraminidase/metabolismo
Trypanosoma cruzi/enzimologia
Animais
Anticorpos Antiprotozoários/sangue
Glicoproteínas
Camundongos Endogâmicos BALB C
Camundongos
Camundongos Nus
Miócitos Cardíacos/citologia
Miócitos Cardíacos/parasitologia
Trypanosoma cruzi/imunologia
Virulência
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