Use este identificador para citar ou linkar para este item: https://www.arca.fiocruz.br/handle/icict/7818

Tipo de documento
Artigo
Direito Autoral
Acesso aberto
Coleções
Compartilhar

Estatísticas
Metadata
Mostrar registro completo

P16(INK4A) EXPRESSION AS A POTENTIAL PROGNOSTIC MARKER IN CERVICAL PRE-NEOPLASTIC AND NEOPLASTIC LESIONS.
Queiroz, Conceição et al. | Data do documento: 2006
Autor(es)
Queiroz, Conceição
Silva, Tânia Maria Correia
Alves, Venâncio Avancini Ferreira
Villa, Luisa L.
Costa, Maria Cecília
Travassos, Ana Gabriela Álvares
Araújo Filho, José Bouzas
Studart, Eduardo
Cheto, Tatiana
Freitas, Luiz Antonio Rodrigues de
Afiliação
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University São Paulo. Department of Pathology. Adolfo Lutz Institute. São Paulo, SP, Brasil
Virology Department. Ludwig Institute for Cancer Research. Sao Paulo, SP, Brasil
Virology Department. Ludwig Institute for Cancer Research. Sao Paulo, SP, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Resumo em Inglês
An immunohistochemical analysis with monoclonal antibody p16INK4a was performed in formalin-fixed, paraffinembedded samples of 60 cases. The aim was to investigate in biopsies the expression of p16INK4a of normal uterine cervical tissue, pre-cancerous and cancerous lesions, and their relation with human papilloma virus (HPV) and HIV status. Three parameters were evaluated: percentage of p16INK4a positive cells, reaction intensity, and cell staining pattern. All of these parameters were statistically different when compared among different histological groups. However, logistic regression model showed that the reaction intensity was the best indicator of the expression of p16INK4a. This expression increases from normal to invasive squamous carcinoma. Sixty-six percent of the patients with CIN grade 1 (CIN1) expressed p16INK4a (all these cases were infected with high risk HPV). Our study supports the hypothesis that p16INK4a expression in pre-cancerous lesions and cancers can be used to identify HPV-transformed cells. Of great interest for routine diagnostic use is the fact that immunohistochemical testing for p16INK4a seems to be capable of identifying HPV-positive cells and potentially recognizing those lesions with an increased risk of progression to high-grade lesions
Palavras-chave em inglês
Cervical neoplasia
Diagnostic marker
p16INK4a
Human papillomavirus; Human immunodeficiency virus
 
DeCS
Neoplasia Intraepitelial Cervical/diagnóstico
Inibidor p16 de Quinase Ciclina-Dependente/metabolismo
Lesões Pré-Cancerosas/diagnóstico
Marcadores Biológicos de Tumor/análise
Neoplasias do Colo do Útero/diagnóstico
Carcinoma/diagnóstico
Neoplasia Intraepitelial Cervical/complicações
Feminino
Colo do Útero/metabolismo
Infecções por HIV/complicações
Infecções por HIV/diagnóstico
Humanos
Imuno-Histoquímica
Infecções por Papillomavirus/complicações
Infecções por Papillomavirus/diagnóstico
Prognóstico
 
Editor
Elsevier
Referência
QUEIROZ, C. et al. P16(INK4a) expression as a potential prognostic marker in cervical pre-neoplastic and neoplastic lesions. Pathology Research Practice, v. 202, n. 2, p. 77-83, 2006.
DOI
10.1016/j.prp.2005.08.012
ISSN
0344-0338