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https://www.arca.fiocruz.br/handle/icict/7569
EVALUATION OF RECOMBINANT LEPTOSPIRA ANTIGEN-BASED ENZYME-LINKED IMMUNOSORBENT ASSAYS FOR THE SERODIAGNOSIS OF LEPTOSPIROSIS.
Antígenos de Bactérias/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Leptospira/imunologia
Leptospirose/diagnóstico
Antígenos de Bactérias/genética
Ensaio de Imunoadsorção Enzimática/normas
Humanos
Imunoglobulina G/sangue
Lipoproteínas/genética
Lipoproteínas/imunologia
Proteínas Recombinantes/genética
Sensibilidade e Especificidade
Author
Affilliation
School of Public Health. University of California. Berkeley, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / School of Pharmacy. Federal University of Bahia. Salvador, BA, Brasil
Division of Infectious Diseases. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California / Department of Medicine. UCLA School of Medicine. Los Angeles, California
Oswaldo Cruz Foundation. Biomanguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil
Oswaldo Cruz Foundation. Biomanguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil
School of Public Health. University of California. Berkeley, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Division of Infectious Diseases. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California / Department of Medicine. UCLA School of Medicine. Los Angeles, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, New York
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / School of Pharmacy. Federal University of Bahia. Salvador, BA, Brasil
Division of Infectious Diseases. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California / Department of Medicine. UCLA School of Medicine. Los Angeles, California
Oswaldo Cruz Foundation. Biomanguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil
Oswaldo Cruz Foundation. Biomanguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil
School of Public Health. University of California. Berkeley, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Division of Infectious Diseases. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California / Department of Medicine. UCLA School of Medicine. Los Angeles, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, New York
Abstract
There is an urgent need for development of new serodiagnostic strategies for leptospirosis, an emerging zoonosis with worldwide distribution. We have evaluated the diagnostic utility of five recombinant antigens in enzyme-linked immunosorbent assays (ELISAs) for serodiagnosis of leptospirosis. Sera from 50 healthy residents of a high-incidence region were used to determine cutoff values for 96% specificity. In paired sera from 50 cases of leptospirosis confirmed by the microscopic agglutination test, immunoglobulin G (IgG) but not IgM reacted with the recombinant leptospiral proteins. The recombinant LipL32 IgG ELISA had the highest sensitivities in the acute (56%) and convalescent (94%) phases of leptospirosis. ELISAs based on recombinant OmpL1, LipL41, and Hsp58 had sensitivities of 16, 24, and 18% during the acute phase and 72, 44, and 32% during convalescence, respectively. Compared to sera from healthy individuals, patient sera did not react significantly with recombinant LipL36 (P > 0.05). Recombinant LipL32 IgG ELISA demonstrated 95% specificity among 100 healthy individuals, and specificities ranging from 90 to 97% among 30 dengue patients, 30 hepatitis patients, and 16 patients with diseases initially thought to be leptospirosis. Among 39 Venereal Disease Research Laboratory test-positive individuals and 30 Lyme disease patients, 13 and 23% of sera, respectively, reacted positively with the rLipL32 antigen. These findings indicate that rLipL32 may be an useful antigen for the serodiagnosis of leptospirosis.
DeCS
Anticorpos Antibacterianos/sangueAntígenos de Bactérias/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Leptospira/imunologia
Leptospirose/diagnóstico
Antígenos de Bactérias/genética
Ensaio de Imunoadsorção Enzimática/normas
Humanos
Imunoglobulina G/sangue
Lipoproteínas/genética
Lipoproteínas/imunologia
Proteínas Recombinantes/genética
Sensibilidade e Especificidade
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