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https://www.arca.fiocruz.br/handle/icict/7413
CD4+CD25+ T CELLS IN SKIN LESIONS OF PATIENTS WITH CUTANEOUS LEISHMANIASIS EXHIBIT PHENOTYPIC AND FUNCTIONAL CHARACTERISTICS OF NATURAL REGULATORY T CELLS.
Leishmania braziliensis
Leishmaniose Cutânea/imunologia
Receptores de Interleucina-2/análise
Pele/imunologia
Linfócitos T Reguladores/imunologia
Adolescente
Adulto
Idoso
Animais
Quimiocinas CC/metabolismo
Criança
Feminino
Fatores de Transcrição Forkhead/metabolismo
Humanos
Imunossupressão
Interleucina-10/metabolismo
Leishmaniose Cutânea/patologia
Ativação Linfocitária
Masculino
Meia-Idade
Fenótipo
Receptores CCR4
Receptores de Quimiocinas/metabolismo
Pele/microbiologia
Pele/patologia
Linfócitos T Reguladores/citologia
Fator de Crescimento Transformador beta/metabolismo
Author
Affilliation
University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology and Division of Dermatology. Ribeirão Preto, SP, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Division of Dermatology. Ribeirão Preto, SP, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology and Division of Dermatology. Ribeirão Preto, SP, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology and Division of Dermatology. Ribeirão Preto, SP, Brasil
Universidade Federal de São Paulo. Department of Microbiology, Immunology, and Parasitology. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology. Salvador, BA, Brasil
Center for Dermatology Dona Libânia. Fortaleza, CE, Brasil
National Institutes of Health. Mucosal Immunology Unit. Laboratory of Parasitic Diseases. Bethesda, MD
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology. Salvador, BA, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology and Division of Dermatology. Ribeirão Preto, SP, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Division of Dermatology. Ribeirão Preto, SP, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology and Division of Dermatology. Ribeirão Preto, SP, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology and Division of Dermatology. Ribeirão Preto, SP, Brasil
Universidade Federal de São Paulo. Department of Microbiology, Immunology, and Parasitology. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology. Salvador, BA, Brasil
Center for Dermatology Dona Libânia. Fortaleza, CE, Brasil
National Institutes of Health. Mucosal Immunology Unit. Laboratory of Parasitic Diseases. Bethesda, MD
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology. Salvador, BA, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology and Division of Dermatology. Ribeirão Preto, SP, Brasil
Abstract
Endogenous regulatory T (Treg) cells are involved in the control of infections, including Leishmania infection
in mice. Leishmania viannia braziliensis is the main etiologic agent of cutaneous leishmaniasis (CL) in Brazil,
and it is also responsible for the more severe mucocutaneous form. Here, we investigated the possible involvement
of Treg cells in the control of the immune response in human skin lesions caused by L. viannia
braziliensis infection. We show that functional Treg cells can be found in skin lesions of patients with CL. These
cells express phenotypic markers of Treg cells—such as CD25, cytotoxic T lymphocyte–associated antigen 4,
Foxp3, and glucocorticoid-induced tumor necrosis factor receptor—and are able to produce large amounts
of interleukin-10 and transforming growth factor–b. Furthermore, CD4+CD25+ T cells derived from the skin
lesions of 4 of 6 patients with CL significantly suppressed in vitro the phytohemagglutinin-inducedproliferative
T cell responses of allogeneic peripheral-blood mononuclear cells (PBMCs) from healthy control subjects at
a ratio of 1 Treg cell to 10 allogeneic PBMCs. These findings suggest that functional Treg cells accumulate at
sites of Leishmania infection in humans and possibly contribute to the local control of effector T cell functions
DeCS
Antígenos CD4/análiseLeishmania braziliensis
Leishmaniose Cutânea/imunologia
Receptores de Interleucina-2/análise
Pele/imunologia
Linfócitos T Reguladores/imunologia
Adolescente
Adulto
Idoso
Animais
Quimiocinas CC/metabolismo
Criança
Feminino
Fatores de Transcrição Forkhead/metabolismo
Humanos
Imunossupressão
Interleucina-10/metabolismo
Leishmaniose Cutânea/patologia
Ativação Linfocitária
Masculino
Meia-Idade
Fenótipo
Receptores CCR4
Receptores de Quimiocinas/metabolismo
Pele/microbiologia
Pele/patologia
Linfócitos T Reguladores/citologia
Fator de Crescimento Transformador beta/metabolismo
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