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LEISHMANIA INFANTUM: LIPOPHOSPHOGLYCAN INTRASPECIFIC VARIATION AND INTERACTION WITH VERTEBRATE AND INVERTEBRATE HOSTS.
Lipophosphoglycan
Lutzomyia longipalpis
Intraspecific variation
Interaction
Author
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Department of Parasitology. Belo Horizonte, MG, Brazil.
University of Kentucky Medical Center. Department of Biochemistry. Lexington, KY, USA.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
University of Kentucky Medical Center. Department of Biochemistry. Lexington, KY, USA
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Federal University of Minas Gerais. Department of Parasitology. Belo Horizonte, MG, Brazil.
University of Kentucky Medical Center. Department of Biochemistry. Lexington, KY, USA.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
University of Kentucky Medical Center. Department of Biochemistry. Lexington, KY, USA
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratory of Medical Entomology. Belo Horizonte, MG, Brazil.
Abstract
Interspecies variations in lipophosphoglycan (LPG) have been the focus of intense study over the years due its role in specificity during sand fly-Leishmaniainteraction. This cell surface glycoconjugate is highly polymorphic among species with variations in sugars that branch off the conserved Gal(b1,4)Man(a1)-PO4backbone of repeat units. However, the degree of intraspecies polymorphism in LPG of Leishmania infantum (syn.Leishmania chagasi) is not known. In this study, intraspecific variation in the repeat units of LPG was evaluated in 16 strains of L. infantum from Brazil, France, Algeria and Tunisia. The structural polymorphism in the L. Infantum LPG repeat units was relatively slight and consisted of three types: type I does not have side chains; type II has oneb-glucose residue that branches off the disaccharide-phosphate repeat units and type III has up to three glucose residues (oligo-glucosylated). The significance of these modifications was investigated during in vivo interaction of L. infantum with Lutzomyia longipalpis,and in vitro interaction of the parasites and respective LPGs with murine macrophages. There were no con-sequential differences in the parasite densities in sand fly midguts infected with Leishmanias trains exhib-iting type I, II and III LPGs. However, higher nitric oxide production was observed in macrophagesexposed to glucosylated type II LPG.
Keywords
Leishmania infantumLipophosphoglycan
Lutzomyia longipalpis
Intraspecific variation
Interaction
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