Please use this identifier to cite or link to this item:
ENHANCING SCAFFOLD-FREE SPHEROID MODELS: 3D CELL BIOPRINTING METHOD FOR METASTATIC HSC3- ORAL SQUAMOUS CARCINOMA CELL LINE
Esferoides
Cultura de células 3D
Protocolos
Bioimpressão magnética
Esferoides celulares
Técnicas de cultura de células em três dimensões
Protocolos clínicos
Bioimpressão
Author
Affilliation
Abstract
3D in vitro systems offer advantages over the shortcomings of two-dimensional models by simulating the morphological and functional features of in vivo-like environments, such as cell-cell and cell-extracellular matrix interactions, as well as the co-culture of different cell types. Nevertheless, these systems present technical challenges that limit their potential in cancer research requiring cell line- and culture-dependent standardization. This protocol details the use of a magnetic 3D bioprinting method and other associated techniques (cytotoxicity assay and histological analysis) using oral squamous cell carcinoma cell line, HSC3, which offer advantages compared to existing widely used approaches. This protocol is particularly timely, as it validates magnetic bioprinting as a method for the rapid deployment of 3D cultures as a tool for compound screening and development of heterotypic cultures such as co-culture of oral squamous cell carcinoma cells with cancer-associated fibroblasts (HSC3/CAFs).
Keywords in Portuguese
Câncer bucalEsferoides
Cultura de células 3D
Protocolos
Bioimpressão magnética
DeCS
Neoplasias bucaisEsferoides celulares
Técnicas de cultura de células em três dimensões
Protocolos clínicos
Bioimpressão
Share