Por favor, use este identificador para citar o enlazar este ítem:
https://www.arca.fiocruz.br/handle/icict/64022
Tipo
ArtículoDerechos de autor
Acceso abierto
Colecciones
Metadatos
Mostrar el registro completo del ítem
AYAHUASCA AND ITS MAJOR COMPONENT HARMINE PROMOTE ANTINOCICEPTIVE EFFECTS IN MOUSE MODELS OF ACUTE AND CHRONIC PAIN
Autor
Afiliación
Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil.
Universidade Federal da Paraíba. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.
Universidade Federal Fluminense. Instituto de Química. Niterói, RJ, Brasil.
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
Universidade Federal da Bahia. Escola de Medicina. Salvador, BA, Brasil.
Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil / Centro Universitário de Tecnologia e Ciência. Escola de Medicina. Salvador, BA, Brasil.
Hospital de Ortopedia e Traumatologia (COT). Salvador, BA, Brasil.
Universidade Federal da Paraíba. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Sistemas Avançados em Saúde. SENAI CIMATEC. Salvador, BA, Brasil.
Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Universidade Federal da Paraíba. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.
Universidade Federal Fluminense. Instituto de Química. Niterói, RJ, Brasil.
Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.
Universidade Federal da Bahia. Escola de Medicina. Salvador, BA, Brasil.
Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil / Centro Universitário de Tecnologia e Ciência. Escola de Medicina. Salvador, BA, Brasil.
Hospital de Ortopedia e Traumatologia (COT). Salvador, BA, Brasil.
Universidade Federal da Paraíba. Departamento de Ciências Farmacêuticas. João Pessoa, PB, Brasil.
Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Sistemas Avançados em Saúde. SENAI CIMATEC. Salvador, BA, Brasil.
Universidade Federal da Bahia. Escola de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Resumen en ingles
Ethnopharmacological relevance: Ayahuasca (AYA) is a psychedelic brew used in religious ceremonies. It is broadly used as a sacred medicine for treating several ailments, including pain of various origins. Aim of the study: To investigate the antinociceptive effects of AYA and its mechanisms in preclinical models of acute and chronic pain in mice, in particular during experimental neuropathy. Materials and methods: The antinociceptive effects of AYA administered orally were assessed in the following models of pain: formalin test, Complete Freund's Adjuvant (CFA)-induced inflammation, tail flick test, and partial sciatic nerve ligation model of neuropathic pain. Antagonism assays and Fos immunohistochemistry in the brain were performed. AYA-induced toxicity was investigated. AYA was chemically characterized. The antinociceptive effect of harmine, the major component present in AYA, was investigated.
Results: AYA (24–3000 μL/kg) dose-dependently reduced formalin-induced pain-like behaviors and CFA-induced mechanical allodynia but did not affect CFA-induced paw edema or tail flick latency. During experimental neuropathy, single treatments with AYA (24–3000 μL/kg) reduced mechanical allodynia; daily treatments once or twice a day for 14 days promoted consistent and sustained antinociception. The antinociceptive effect of AYA (600 μL/kg) was reverted by bicuculline (1 mg/kg) and methysergide (5 mg/kg), but not by naloxone (5 mg/kg), phaclofen (2 mg/kg), and rimonabant (10 mg/kg), suggesting the roles of GABAA and serotonergic receptors. AYA increased Fos expression in the ventrolateral periaqueductal gray and nucleus raphe magnus after 1 h, but not after 6 h or 14 days of daily treatments. AYA (600 μL/kg) twice a day for 14 days did not alter mice's motor function, spontaneous locomotion, body weight, food and water intake, hematological, biochemical, and histopathological parameters. Harmine (3.5 mg/kg) promoted consistent antinociception during experimental neuropathy. Conclusions: AYA promotes consistent antinociceptive effects in different mouse models of pain without inducing detectable toxic effects. Harmine is at least partially accountable for the antinociceptive properties of AYA.
Compartir