Use este identificador para citar ou linkar para este item:
https://www.arca.fiocruz.br/handle/icict/62990
DEVELOPMENT OF A QUANTITATIVE NS1 ANTIGEN ENZYME‑LINKED IMMUNOSORBENT ASSAY (ELISA) FOR ZIKA VIRUS DETECTION USING A NOVEL VIRUS‑SPECIFIC MAB
Reagent Kits, Diagnostic
Enzyme-Linked Immunosorbent Assay
NS1
Juego de Reactivos para Diagnóstico
Ensayo de Inmunoadsorción Enzimática
NS1
Proteínas não Estruturais Virais
Kit de Reagentes para Diagnóstico
Ensaio de Imunoadsorção Enzimática
Autor(es)
Afiliação
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Viral Gene Expression Group. The Pirbright Institute. Pirbright. Surrey, UK.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Viral Gene Expression Group. The Pirbright Institute. Pirbright. Surrey, UK.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
Resumo em Inglês
Viruses from the Flaviviridae family, such as Dengue virus (DENV), Yellow fever virus (YFV), and Zika virus (ZIKV) are notorious global public health problems. ZIKV emergence in Polynesia and the Americas from 2013 to 2016 raised concerns as new distinguishing features set it apart from previous outbreaks, including its association with neurological complications and heightened disease severityVirus detection is impaired as cross-reactivity to other closely related orthofaviviruses is common among commercially available diagnostic kits. While non-structural protein 1 (NS1) has been used as an early marker of DENV and West Nile virus (WNV) infection, little is known about NS1 expression during ZIKV infection. In the present work, we developed a NS1 capture ELISA using a novel ZIKVspecifc monoclonal antibody to study NS1 expression dynamics in vitro in mosquito and human cell lines. While detectable in culture supernatants, higher concentrations of NS1 were predominantly cell-associated. To our knowledge, this is the frst report of NS1 detection in human cells despite viral clearance over time. Tests with human samples need to be conducted to validate the applicability of NS1 detection for diagnosis, but overall, the tools developed in this work are promising for specifc detection of acute ZIKV infection.
Palavras-chave em inglês
Viral Nonstructural ProteinsReagent Kits, Diagnostic
Enzyme-Linked Immunosorbent Assay
NS1
Palavras-chave em espanhol
Proteínas no Estructurales ViralesJuego de Reactivos para Diagnóstico
Ensayo de Inmunoadsorción Enzimática
NS1
DeCS
Zika virusProteínas não Estruturais Virais
Kit de Reagentes para Diagnóstico
Ensaio de Imunoadsorção Enzimática
Compartilhar