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BIOACTIVE DECELLULARIZED EXTRACELLULAR MATRIX-BASED HYDROGEL SUPPORTS HUMAN ADIPOSE TISSUE-DERIVED STEM CELL MAINTENANCE AND FIBROCARTILAGE PHENOTYPE
Mesenchymal Stem Cells
Regenerative Medicine
Tissue Engineering
Cartilage
Células Madre Mesenquimatosas
Medicina Regenerativa
Ingeniería de Tejidos
Cartílago
Células-Tronco Mesenquimais
Medicina Regenerativa
Engenharia Tecidual
Cartilagem
Afiliación
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Resumen en ingles
Articular cartilage is a highly specialized tissue able to tolerate physical stress. However, its capacity for restoration is restricted, and injuries to the cartilage do not recover spontaneously. Interest in mesenchymal stem cells derived from human adipose tissue (hASCs) is growing due to their potential to improve tissue healing and recovery. Decellularized extracellular matrix (dECM)-based hydrogels combined with hASCs could serve as an interface for studying behavior and differentiation properties in a cartilage microenvironment. In the present study, we described the behavior of hASCs cultured in a commercial dECM MatriXpec™. The structural microtopography of MatriXpec™ was analyzed by scanning electron micrography, and its protein composition was accessed by mass spectrometry. The protein composition of MatriXpec™ is mainly represented by collagen proteins, building its fibrous ultrastructure. hASCs were cultured three-dimensionally (3D) on MatriXpec™ to perform cell viability, growth, and cartilage differentiation analysis. We showed that MatriXpec™ could be loaded with hASCs and that it supports cell maintenance for several days. We observed that the three-dimensional ultrastructure of the biomaterial is composed of nanofibers, and its protein composition reflects the tissue from which it was harvested. Finally, we showed that the molecular cues from the hydrogel are biologically active as these influence cell behavior and differentiation phenotype, increasing the expression of fibrocartilage-related genes such as SOX9, COL1, COL10, and MMP13. MatriXpec™ hydrogel can be used as an interface for 3D hASCs culture studies as it maintains cell viability and supports its differentiation process.
Palabras clave en ingles
Decellularized Extracellular MatrixMesenchymal Stem Cells
Regenerative Medicine
Tissue Engineering
Cartilage
Palabras clave
Matriz Extracelular DescelularizadaCélulas Madre Mesenquimatosas
Medicina Regenerativa
Ingeniería de Tejidos
Cartílago
DeCS
Matriz Extracelular DescelularizadaCélulas-Tronco Mesenquimais
Medicina Regenerativa
Engenharia Tecidual
Cartilagem
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