Por favor, use este identificador para citar o enlazar este ítem:
https://www.arca.fiocruz.br/handle/icict/59379
Tipo
ArtículoDerechos de autor
Acceso restringido
Fecha del embargo
2030-12-31
Colecciones
- CDTS - Artigos de Periódicos [369]
- IOC - Artigos de Periódicos [12596]
Metadatos
Mostrar el registro completo del ítem
MALDI (MATRIX ASSISTED LASER DESORPTION IONIZATION) IMAGING MASS SPECTROMETRY (IMS) OF SKIN: ASPECTS OF SAMPLE PREPARATION
Molecular histology
Pathophysiology
Direct tissue-analysis
Spatial-resolution
Lipid changes
Formalin
MSI
Quantitation
Sensitivity
Metabolites
Absorption
Sections
Afiliación
Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brazil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Cellular Microbiology Laboratory. Rio de Janeiro, RJ, Brazil.
Mass Spectrometry Research Center. Vanderbilt University Medical Center. Nashville, USA.
Mass Spectrometry Research Center. Vanderbilt University Medical Center. Nashville, USA.
Mass Spectrometry Research Center. Vanderbilt University Medical Center. Nashville, USA.
Mass Spectrometry Research Center. Vanderbilt University Medical Center. Nashville, USA.
Resumen en ingles
MALDI (matrix assisted laser desorption ionization) Imaging Mass Spectrometry (IMS) allows molecular analysis of biological materials making possible the identification and localization of molecules in tissues, and has been applied to address many questions on skin pathophysiology, as well as on studies about drug absorption and metabolism. Sample preparation for MALDI IMS is the most important part of the workflow, comprising specimen collection and preservation, tissue embedding, cryosectioning, washing, and matrix application. These steps must be carefully optimized for specific analytes of interest (lipids, proteins, drugs, etc.), representing a challenge for skin analysis. In this review, critical parameters for MALDI IMS sample preparation of skin samples will be described. In addition, specific applications of MALDI IMS of skin samples will be presented including wound healing, neoplasia, and infection.
Palabras clave en ingles
Maldi matricesMolecular histology
Pathophysiology
Direct tissue-analysis
Spatial-resolution
Lipid changes
Formalin
MSI
Quantitation
Sensitivity
Metabolites
Absorption
Sections
Compartir