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ECOLOGICAL, GENETIC, AND PHYLOGENETIC ASPECTS OF YFV 2017–2019 SPREAD IN RIO DE JANEIRO STATE
Ribeiro, Ieda Pereira et al. | Data do documento: 2023
Autor(es)
Ribeiro, Ieda Pereira
Delatorre, Edson
Abreu, Filipe Vieira Santos de
Santos, Alexandre Araújo Cunha dos
Furtado, Nathália Dias
Ferreira-de-Brito, Anielly
Pina-Costa, Anielle de
Neves, Maycon Sebastião Alberto Santos
Castro, Márcia Gonçalves de
Motta, Monique de Albuquerque
Brasil, Patricia
Lourenço-de-Oliveira, Ricardo
Bonaldo, Myrna Cristina
Afiliação
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brazil.
Universidade Federal do Espírito Santo. Centro de Ciências Exatas, Naturais e da Saúde. Laboratório de Genômica Evolutiva e Ambiental, Departamento de Biologia. Vitória, ES, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Instituto Federal do Norte de Minas Gerais. Salinas, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brazil / Faculdade de Medicina de Teresópolis. Centro Universitário Serra dos Órgãos UNIFESO. Teresópolis, RJ, Brazil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.
Resumo em Inglês
In Brazil, a yellow fever (YF) outbreak was reported in areas considered YF-free for decades. The low vaccination coverage and the increasing forest fragmentation, with the wide distribution of vector mosquitoes, have been related to yellow fever virus (YFV) transmission beyond endemic areas since 2016. Aiming to elucidate the molecular and phylogenetic aspects of YFV spread on a local scale, we generated 43 new YFV genomes sampled from humans, non-human primates (NHP), and primarily, mosquitoes from highly heterogenic areas in 15 localities from Rio de Janeiro (RJ) state during the YFV 2016-2019 outbreak in southeast Brazil. Our analysis revealed that the genetic diversity and spatial distribution of the sylvatic transmission of YFV in RJ originated from at least two introductions and followed two chains of dissemination, here named the YFV RJ-I and YFV RJ-II clades. They moved with similar dispersal speeds from the north to the south of the RJ state in parallel directions, separated by the Serra do Mar Mountain chain, with YFV RJ-I invading the north coast of São Paulo state. The YFV RJ-I clade showed a more significant heterogeneity across the entire polyprotein. The YFV RJ-II clade, with only two amino acid polymorphisms, mapped at NS1 (I1086V), present only in mosquitoes at the same locality and NS4A (I2176V), shared by all YFV clade RJ-II, suggests a recent clustering of YFV isolates collected from different hosts. Our analyses strengthen the role of surveillance, genomic analyses of YVF isolated from other hosts, and environmental studies into the strategies to forecast, control, and prevent yellow fever outbreaks.
Palavras-chave em inglês
Brazilian outbreak
Amino acid polymorphisms
Ecology
Epidemiology
Genomic surveillance
Yellow fever virus
 
Editor
MDPI
Referência
RIBEIRO, Ieda Pereira et al. Ecological, Genetic, and Phylogenetic Aspects of YFV 2017-2019 Spread in Rio de Janeiro State. Viruses, v. 15, n. 2, p. 1-17, Feb. 2023.
DOI
10.3390/v15020437
ISSN
1999-4915
Notas
Institutional Review Board Statement: The study was conducted in accordance with the Declaration of Helsinki, and approved by the Institutional Ethics Committee of Instituto Oswaldo Cruz (IOC) (CAAE 69206217.8.0000.5248), which exempted the need for specific written informed consent from patients or their legal representatives. The animal study protocol was approved by the Institutional Ethics Committee of Brazilian Ministry of Environment (SISBIO 41837-3—20 May 2015, 52472-2—26 January 2016, and 54707-5—25 August 2016) and Rio de Janeiro’s Environment Agency (INEA 012/2016—07 February 2016 and 019/2018—19 April 2018) and by the institutional Ethics Committee for Animal Experimentation (protocol CEUA/IOC-004/2015—10 April 2015, licenseL037/2016—24 August 2016). Informed Consent Statement: Patient consent was waived due to the exemption of the Ethics Committee of Instituto Oswaldo Cruz. Data Availability Statement: Not applicable. Acknowledgments: To Gonzalo Bello for the inspiring insights, valuable discussions of methodological approaches and results throughout the outbreak, and study design. To Romário Gabriel Aquino (Environmental Surveillance, Secretaria Municipal de Saúde de Angra dos Reis), Tercius Barrada (Parque Estadual da Ilha Grande), Mário Sérgio Ribeiro and Patrícia Menegueti (Secretaria de Saúde do Estado do Rio de Janeiro), Prefeitura de Macaé, Centro de Estudos Ambientais e Desenvolvimento Sustentável (CEADS/UERJ), Marcelo Celestino dos Santos, Mauro M. Muniz, and Marcelo Q. Gomes for the help in obtaining the viral samples and support in the fieldwork. We are grateful to the Plataforma Genômica—Sequenciamento de DNA-RPT01A—Rede de Plataformas Tecnológicas FIOCRUZ for the services provided in the genome sequencing. We also thank Heloísa Diniz from Serviço de Produção e Tratamento de Imagens, IOC, Fiocruz, for the design of the figures.