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2099-12-31
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IMPROVEMENT OF DIRECT AGGLUTINATION TEST (DAT) FOR LABORATORY DIAGNOSIS OF VISCERAL LEISHMANIASIS IN BRAZIL
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Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil.
Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Pesquisa Clinica. Belo Horizonte, MG, Brazil.
Abstract
We previously standardized the direct agglutination test (DAT) to detect antiLeishmania chagasi promastigote antibodies (DAT-LPC) with good sensitivity and specificity for diagnosing visceral. leishmaniasis (VL). In this paper, we present a technical upgrade by introducing some modifications into the antigen preparation. This antigen was evaluated in DAT (DAT-Mod) using 61 sera samples from VL patients and 96 samples from patients with other diseases. The DAT-Mod presented a cut-off of 1:100, satisfactory reproducibility (VC <5.8), sensitivity of 93.4%, specificity of 96.9%, and diagnostic efficiency of 95.5%. The improvement in antigen preparation reduced inter-batch variations and resulted in a high test performance. (C) 2009 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved
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