Use este identificador para citar ou linkar para este item:
ACCURATE, FAST AND COST-EFFECTIVE SIMULTANEOUS DETECTION OF BACTERIAL MENINGITIS BY QUALITATIVE PCR WITH HIGH-RESOLUTION MELTING
Autor(es)
Afiliação
Resumo em Inglês
Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae are leading causes of meningitis and acute invasive infections. PCR-based methods are widely used for the diagnosis and surveillance of bacterial pathogens because of their high sensitivity, specificity and high-throughput capabilities compared with conventional laboratory methods. This study evaluated a high-resolution melting qualitative PCR analysis method for the simultaneous detection of these three pathogens. The assay has been optimized to detect three species-specific genes of each organism isolated from clinical samples, enabling accurate identification of the etiological agent. The method proved to be highly sensitive and cheaper than the real-time PCR TaqManR system because it is probe-free; it could be used for the diagnosis of invasive diseases in public health laboratories of developing countries.
Compartilhar