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ACCURATE, FAST AND COST-EFFECTIVE SIMULTANEOUS DETECTION OF BACTERIAL MENINGITIS BY QUALITATIVE PCR WITH HIGH-RESOLUTION MELTING
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Abstract
Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae are leading causes of meningitis and acute invasive infections. PCR-based methods are widely used for the diagnosis and surveillance of bacterial pathogens because of their high sensitivity, specificity and high-throughput capabilities compared with conventional laboratory methods. This study evaluated a high-resolution melting qualitative PCR analysis method for the simultaneous detection of these three pathogens. The assay has been optimized to detect three species-specific genes of each organism isolated from clinical samples, enabling accurate identification of the etiological agent. The method proved to be highly sensitive and cheaper than the real-time PCR TaqManR system because it is probe-free; it could be used for the diagnosis of invasive diseases in public health laboratories of developing countries.
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