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A NEW STRATEGY FOR MAPPING EPITOPES OF LACK AND PEPCK PROTEINS OF LEISHMANIA AMAZONENSIS SPECIFIC FOR MAJOR HISTOCOMPATIBILITY COMPLEX CLASS I
Epítopos T
Peptídeos
Vacina
Bioinformática
HLA
H2Db
MHC I
Tetrâmero
Tepitopes
Peptides
Vaccine
Bioinformatics
HLA
H2Db
MHC I
Tetramer
Autor
Afiliación
Resumen en ingles
Leishmaniasis represents a complex of diseases with a broad clinical spectrum and epidemiological
diversity, considered a major public health problem. Although there is treatment, there are
still no vaccines for cutaneous leishmaniasis. Because Leishmania spp. is an intracellular protozoan
with several escape mechanisms, a vaccine must provoke cellular and humoral immune responses.
Previously, we identified the Leishmania homolog of receptors for activated C kinase (LACK) and
phosphoenolpyruvate carboxykinase (PEPCK) proteins as strong immunogens and candidates for the
development of a vaccine strategy. The present work focuses on the in silico prediction and characterization
of antigenic epitopes that might interact with mice or human major histocompatibility complex
class I. After immunogenicity prediction on the Immune Epitope Database (IEDB) and the Database of
MHC Ligands and Peptide Motifs (SYFPEITHI), 26 peptides were selected for interaction assays with
infected mouse lymphocytes by flow cytometry and ELISpot. This strategy identified nine antigenic
peptides (pL1-H2, pPL3-H2, pL10-HLA, pP13-H2, pP14-H2, pP15-H2, pP16-H2, pP17-H2, pP18-H2,
pP26-HLA), which are strong candidates for developing a peptide vaccine against leishmaniasis.
Palabras clave en portugues
Leishmaniose cutâneaEpítopos T
Peptídeos
Vacina
Bioinformática
HLA
H2Db
MHC I
Tetrâmero
Palabras clave en ingles
Cutaneous leishmaniasisTepitopes
Peptides
Vaccine
Bioinformatics
HLA
H2Db
MHC I
Tetramer
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