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https://www.arca.fiocruz.br/handle/icict/57035
PLASMODIUM INFECTION IS ASSOCIATED WITH CROSS‑REACTIVE ANTIBODIES TO CARBOHYDRATE EPITOPES ON THE SARS‑COV‑2 SPIKE PROTEIN
Author
Lapidus, Sarah
Liu, Feimei
Massana, Arnau Casanovas
Dai, Yile
Huck, John D.
Lucas, Carolina
Klein, Jon
Filler, Renata B.
Strine, Madison S.
Sy, Mouhamad
Deme, Awa B.
Badiane, Aida S.
Dieye, Baba
Ndiaye, Ibrahima Mbaye
Diedhiou, Younous
Mbaye, Amadou Moctar
Diagne, Cheikh Tidiane
Womas, Ines Vigan
Mbengue, Alassane
Sadio, Bacary D.
Diagne, Moussa M.
Moore, Adam J.
Mangou, Khadidiatou
Diallo, Fatoumata
Sene, Seynabou D.
Pouye, Mariama N.
Faye, Rokhaya
Diouf, Babacar
Nery Jr, Nivison
Costa, Federico
Reis, Mitermayer
Muenker, M. Catherine
Hodson, Daniel Z.
Mbarga, Yannick
Katz, Ben Z.
Andrews, Jason R.
Campbell, Melissa
Srivathsan, Ariktha
Kamath, Kathy
Jones, Elisabeth Baum
Faye, Ousmane
Sall, Amadou Alpha
Velez, Juan Carlos Quintero
Cappello, Michael
Wilson, Michael
Mamoun, Choukri Ben
Tedder, Richard
McClure, Myra
Cherepanov, Peter
Somé, Fabrice A.
Dabiré, Roch K.
Moukoko, Carole Else Eboumbou
Ouédraogo, Jean Bosco
Yap, Boum II
Shon, John
Ndiaye, Daouda
Wisnewski, Adam
Parikh, Sunil
Iwasaki, Akiko
Wilen, Craig B.
Ko, Albert I.
Ring, Aaron M.
Bei, Amy K.
Liu, Feimei
Massana, Arnau Casanovas
Dai, Yile
Huck, John D.
Lucas, Carolina
Klein, Jon
Filler, Renata B.
Strine, Madison S.
Sy, Mouhamad
Deme, Awa B.
Badiane, Aida S.
Dieye, Baba
Ndiaye, Ibrahima Mbaye
Diedhiou, Younous
Mbaye, Amadou Moctar
Diagne, Cheikh Tidiane
Womas, Ines Vigan
Mbengue, Alassane
Sadio, Bacary D.
Diagne, Moussa M.
Moore, Adam J.
Mangou, Khadidiatou
Diallo, Fatoumata
Sene, Seynabou D.
Pouye, Mariama N.
Faye, Rokhaya
Diouf, Babacar
Nery Jr, Nivison
Costa, Federico
Reis, Mitermayer
Muenker, M. Catherine
Hodson, Daniel Z.
Mbarga, Yannick
Katz, Ben Z.
Andrews, Jason R.
Campbell, Melissa
Srivathsan, Ariktha
Kamath, Kathy
Jones, Elisabeth Baum
Faye, Ousmane
Sall, Amadou Alpha
Velez, Juan Carlos Quintero
Cappello, Michael
Wilson, Michael
Mamoun, Choukri Ben
Tedder, Richard
McClure, Myra
Cherepanov, Peter
Somé, Fabrice A.
Dabiré, Roch K.
Moukoko, Carole Else Eboumbou
Ouédraogo, Jean Bosco
Yap, Boum II
Shon, John
Ndiaye, Daouda
Wisnewski, Adam
Parikh, Sunil
Iwasaki, Akiko
Wilen, Craig B.
Ko, Albert I.
Ring, Aaron M.
Bei, Amy K.
Affilliation
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA / Department of Laboratory Medicine. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA / Department of Laboratory Medicine. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
DiaTROPIX Rapid Diagnostic Tests Facility. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Virologie. Institut Pasteur de Dakar. Dakar, Senegal.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil / Department of Internal Medicine, Yale Occupational and Environmental Medicine Program. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil / Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Douala Military Hospital. Douala, Cameroon.
Division of Infectious Diseases. Department of Pediatrics. Northwestern University Feinberg School of Medicine. Ann & Robert H. Lurie Children’s Hospital of Chicago. Chicago, USA.
Division of Infectious Diseases and Geographic Medicine. School of Medicine. Stanford University. Stanford, CA, USA.
Yale Center for Clinical Investigation. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Serimmune. Inc. Goleta, CA, USA.
Serimmune. Inc. Goleta, CA, USA.
Pôle Virologie. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Virologie. Institut Pasteur de Dakar. Dakar, Senegal.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Grupo de Investigación Ciencias Veterinarias Centauro. University of Antioquia. Medellín, Colombia /Grupo de Investigación Microbiología Básica y aplicada. University of Antioquia. Medellín, Colombia.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Department of Pediatrics. Yale School of Medicine. New Haven, CT, USA.
Department of Parasitology. Noguchi Memorial Institute for Medical Research. University of Ghana. Accra, Ghana.
Department of Internal Medicine. Section of Infectious Diseases. Yale School of Medicine. New Haven, CT, USA.
Department of Infectious Disease. Imperial College London. St Mary’s Campus. London, UK. / South London Specialist Virology Centre. Kings College Hospital NHS Foundation Trust. London, UK.
Department of Infectious Disease. Imperial College London. St Mary’s Campus. London, UK.
Department of Infectious Disease. Imperial College London. St Mary’s Campus. London, UK / Chromatin Structure and Mobile DNA Laboratory. The Francis Crick Institute. London, UK /Crick COVID19 Consortium. Francis Crick Institute. London, UK.
Institut de Recherche en Sciences de La Santé (IRSS)/Centre Muraz. Bobo Dioulasso. Burkina Faso.
Institut de Recherche en Sciences de La Santé (IRSS)/Centre Muraz. Bobo Dioulasso. Burkina Faso.
Department of Biological Sciences. Faculty of Medicine and Pharmaceutical Sciences. University of Douala. Douala, BP, Cameroon / Malaria Research Unit. Center Pasteur Cameroon. Yaoundé, Cameroon.
Institut de Recherche en Sciences de La Santé (IRSS)/Centre Muraz. Bobo Dioulasso. Burkina Faso.
Médecins Sans Frontières. University of Yaoundé and Epicentre. Yaoundé, Cameroon.
Serimmune. Inc. Goleta, CA, USA.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Department of Internal Medicine, Yale Occupational and Environmental Medicine Program. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA / Howard Hughes Medical Institute. Chevy Chase, MD, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal / G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA / Department of Laboratory Medicine. Yale School of Medicine. New Haven, CT, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA / Department of Laboratory Medicine. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
DiaTROPIX Rapid Diagnostic Tests Facility. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Virologie. Institut Pasteur de Dakar. Dakar, Senegal.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil / Department of Internal Medicine, Yale Occupational and Environmental Medicine Program. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil / Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Douala Military Hospital. Douala, Cameroon.
Division of Infectious Diseases. Department of Pediatrics. Northwestern University Feinberg School of Medicine. Ann & Robert H. Lurie Children’s Hospital of Chicago. Chicago, USA.
Division of Infectious Diseases and Geographic Medicine. School of Medicine. Stanford University. Stanford, CA, USA.
Yale Center for Clinical Investigation. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Serimmune. Inc. Goleta, CA, USA.
Serimmune. Inc. Goleta, CA, USA.
Pôle Virologie. Institut Pasteur de Dakar. Dakar, Senegal.
Pôle Virologie. Institut Pasteur de Dakar. Dakar, Senegal.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Grupo de Investigación Ciencias Veterinarias Centauro. University of Antioquia. Medellín, Colombia /Grupo de Investigación Microbiología Básica y aplicada. University of Antioquia. Medellín, Colombia.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Department of Pediatrics. Yale School of Medicine. New Haven, CT, USA.
Department of Parasitology. Noguchi Memorial Institute for Medical Research. University of Ghana. Accra, Ghana.
Department of Internal Medicine. Section of Infectious Diseases. Yale School of Medicine. New Haven, CT, USA.
Department of Infectious Disease. Imperial College London. St Mary’s Campus. London, UK. / South London Specialist Virology Centre. Kings College Hospital NHS Foundation Trust. London, UK.
Department of Infectious Disease. Imperial College London. St Mary’s Campus. London, UK.
Department of Infectious Disease. Imperial College London. St Mary’s Campus. London, UK / Chromatin Structure and Mobile DNA Laboratory. The Francis Crick Institute. London, UK /Crick COVID19 Consortium. Francis Crick Institute. London, UK.
Institut de Recherche en Sciences de La Santé (IRSS)/Centre Muraz. Bobo Dioulasso. Burkina Faso.
Institut de Recherche en Sciences de La Santé (IRSS)/Centre Muraz. Bobo Dioulasso. Burkina Faso.
Department of Biological Sciences. Faculty of Medicine and Pharmaceutical Sciences. University of Douala. Douala, BP, Cameroon / Malaria Research Unit. Center Pasteur Cameroon. Yaoundé, Cameroon.
Institut de Recherche en Sciences de La Santé (IRSS)/Centre Muraz. Bobo Dioulasso. Burkina Faso.
Médecins Sans Frontières. University of Yaoundé and Epicentre. Yaoundé, Cameroon.
Serimmune. Inc. Goleta, CA, USA.
Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal.
Department of Internal Medicine, Yale Occupational and Environmental Medicine Program. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA / Howard Hughes Medical Institute. Chevy Chase, MD, USA.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Ministério da Saúde. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.
Department of Immunobiology. Yale School of Medicine. New Haven, CT, USA.
Department of Epidemiology of Microbial Diseases. Yale School of Public Health. New Haven, CT, USA / Laboratory of Parasitology and Mycology. Aristide le Dantec Hospital. Cheikh Anta Diop University. Dakar, Senegal / G4 Malaria Experimental Genetic Approaches and Vaccines. Pôle Immunophysiopathologie et Maladies Infectieuses. Institut Pasteur de Dakar. Dakar, Senegal.
Abstract
Sero-surveillance can monitor and project disease burden and risk. However, SARS-CoV-2 antibody test results can produce false positive results, limiting their efficacy as a sero-surveillance tool. False positive SARS-CoV-2 antibody results are associated with malaria exposure, and understanding this association is essential to interpret sero-surveillance results from malaria-endemic countries. Here, pre-pandemic samples from eight malaria endemic and non-endemic countries and four continents were tested by ELISA to measure SARS-CoV-2 Spike S1 subunit reactivity. Individuals with acute malaria infection generated substantial SARS-CoV-2 reactivity. Cross-reactivity was not associated with reactivity to other human coronaviruses or other SARS-CoV-2 proteins, as measured by peptide and protein arrays. ELISAs with deglycosylated and desialated Spike S1 subunits revealed that cross-reactive antibodies target sialic acid on N-linked glycans of the Spike protein. The functional activity of cross-reactive antibodies measured by neutralization assays showed that cross-reactive antibodies did not neutralize SARS-CoV-2 in vitro. Since routine use of glycosylated or sialated assays could result in false positive SARS-CoV-2 antibody results in malaria endemic regions, which could overestimate exposure and population-level immunity, we explored methods to increase specificity by reducing cross-reactivity. Overestimating population-level exposure to SARS-CoV-2 could lead to underestimates of risk of continued COVID-19 transmission in sub-Saharan Africa.
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