Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/56561
EX-VIVO MUCOLYTIC AND ANTI-INFLAMMATORY ACTIVITY OF BROMAC IN TRACHEAL ASPIRATES FROM COVID-19
Author
Reis, Jordana Grazziela Alves Coelho dos
Ferreira, Geovane Marques
Lourenço, Alice Aparecida
Ribeiro, Ágata Lopes
Mata, Camila Pacheco da Silveira Martins da
Oliveira, Patrícia de Melo
Marques, Daisymara Priscila de Almeida
Ferreira, Linziane Lopes
Clarindo, Felipe Alves
Silva, Murillo Ferreira da
Póvoas Filho, Heitor Portella
Oliveira Junior, Nilson Roberto Ribeiro
Sodré, Maisah Meyhr D'Carmo
Gadelha, Sandra Rocha
Albuquerque, George Rego
Maciel, Bianca Mendes
Mariano, Ana Paula Melo
Silva, Mylene de Melo
Fontana, Renato
Marin, Lauro Juliano
Carlos, Renata Santiago Alberto
Lopes, Amanda Teixeira Sampaio
Ferreira, Fabrício Barbosa
Santos, Uener Ribeiro Dos
Santana, Íris Terezinha Santos de
Fehlberg, Hllytchaikra Ferraz
Rezende, Rachel Passos
Dias, João Carlos T
Gross, Eduardo
Goulart, Gisele Assis Castro
Santiago, Marie Gabriele
Lemos, Ana Paula Motta Lavigne de
Conceição, Aline O da
Romano, Carla Cristina
Carvalho, Luciana Debortoli de
Martins Filho, Olindo Assis
Quadros, Claudio Almeida
Morris, David L
Valle, Sarah J
Ferreira, Geovane Marques
Lourenço, Alice Aparecida
Ribeiro, Ágata Lopes
Mata, Camila Pacheco da Silveira Martins da
Oliveira, Patrícia de Melo
Marques, Daisymara Priscila de Almeida
Ferreira, Linziane Lopes
Clarindo, Felipe Alves
Silva, Murillo Ferreira da
Póvoas Filho, Heitor Portella
Oliveira Junior, Nilson Roberto Ribeiro
Sodré, Maisah Meyhr D'Carmo
Gadelha, Sandra Rocha
Albuquerque, George Rego
Maciel, Bianca Mendes
Mariano, Ana Paula Melo
Silva, Mylene de Melo
Fontana, Renato
Marin, Lauro Juliano
Carlos, Renata Santiago Alberto
Lopes, Amanda Teixeira Sampaio
Ferreira, Fabrício Barbosa
Santos, Uener Ribeiro Dos
Santana, Íris Terezinha Santos de
Fehlberg, Hllytchaikra Ferraz
Rezende, Rachel Passos
Dias, João Carlos T
Gross, Eduardo
Goulart, Gisele Assis Castro
Santiago, Marie Gabriele
Lemos, Ana Paula Motta Lavigne de
Conceição, Aline O da
Romano, Carla Cristina
Carvalho, Luciana Debortoli de
Martins Filho, Olindo Assis
Quadros, Claudio Almeida
Morris, David L
Valle, Sarah J
Affilliation
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Risoleta Tolentino Neves Hospital. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Hospital de Ilhéus. Ilhéus, BA, Brazil.
Hospital de Ilhéus. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil/Department of Agricultural and Environmental Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil/Department of Health Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Agricultural and Environmental Science. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Pharmaceuticals. Faculty of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Pharmaceuticals. Faculty of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil.
Hospital de Ilhéus. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Hospital de Ilhéus. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Grupo Integrado de Pesquisas em Biomarcadores. Instituto René Rachou. Belo Horizonte, MG, Brazil.
São Rafael Hospital. Salvador, Bahia, Brazil/Bahia State University. Salvador, Bahia, Brazil.
Mucpharm Pty Ltd. Sydney, NSW, Australia/University of New South Wales. St George & Sutherland Hospital Clinical School. Sydney, NSW, Australia/Department of Surgery. St George Hospital, Sydney.NSW, Australia.
Mucpharm Pty Ltd. Sydney, NSW, Australia/University of New South Wales. St George & Sutherland Hospital Clinical School. Sydney, NSW, Australia.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Risoleta Tolentino Neves Hospital. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Basic and Applied Virology Laboratory. Department of Microbiology. Institute for Biological Sciences. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Hospital de Ilhéus. Ilhéus, BA, Brazil.
Hospital de Ilhéus. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil/Department of Agricultural and Environmental Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil/ Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil/Department of Health Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Agricultural and Environmental Science. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Laboratory of Pharmacogenomics and Molecular Epidemiology. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Department of Pharmaceuticals. Faculty of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil.
Department of Pharmaceuticals. Faculty of Pharmacy. Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brazil.
Hospital de Ilhéus. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Hospital de Ilhéus. Ilhéus, BA, Brazil.
Department of Biological Sciences. Santa Cruz State University. Ilhéus, BA, Brazil.
Grupo Integrado de Pesquisas em Biomarcadores. Instituto René Rachou. Belo Horizonte, MG, Brazil.
São Rafael Hospital. Salvador, Bahia, Brazil/Bahia State University. Salvador, Bahia, Brazil.
Mucpharm Pty Ltd. Sydney, NSW, Australia/University of New South Wales. St George & Sutherland Hospital Clinical School. Sydney, NSW, Australia/Department of Surgery. St George Hospital, Sydney.NSW, Australia.
Mucpharm Pty Ltd. Sydney, NSW, Australia/University of New South Wales. St George & Sutherland Hospital Clinical School. Sydney, NSW, Australia.
Abstract
COVID-19 is a lethal disease caused by the pandemic SARS-CoV-2, which continues to be a public health threat. COVID-19 is principally a respiratory disease and is often associated with sputum retention and cytokine storm, for which there are limited therapeutic options. In this regard, we evaluated the use of BromAc®, a combination of Bromelain and Acetylcysteine (NAC). Both drugs present mucolytic effect and have been studied to treat COVID-19. Therefore, we sought to examine the mucolytic and anti-inflammatory effect of BromAc® in tracheal aspirate samples from critically ill COVID-19 patients requiring mechanical ventilation.
Method: Tracheal aspirate samples from COVID-19 patients were collected following next of kin consent and mucolysis, rheometry and cytokine analysis using Luminex kit was performed.
Results: BromAc® displayed a robust mucolytic effect in a dose dependent manner on COVID-19 sputum ex vivo. BromAc® showed anti-inflammatory activity, reducing the action of cytokine storm, chemokines including MIP-1alpha, CXCL8, MIP-1b, MCP-1 and IP-10, and regulatory cytokines IL-5, IL-10, IL-13 IL-1Ra and total reduction for IL-9 compared to NAC alone and control. BromAc® acted on IL-6, demonstrating a reduction in G-CSF and VEGF-D at concentrations of 125 and 250 µg.
Conclusion: These results indicate robust mucolytic and anti-inflammatory effect of BromAc® ex vivo in tracheal aspirates from critically ill COVID-19 patients, indicating its potential to be further assessed as pharmacological treatment for COVID-19.
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